Division of Crop Science, ICAR Research Complex for NEH Region, Barapani, Meghalaya, India.
International Centre for Genetic Engineering and Biotechnology (ICGEB), New Delhi, India.
Sci Rep. 2023 Jul 27;13(1):12184. doi: 10.1038/s41598-023-39238-8.
Aluminium (Al) is the third most abundant element in the Earth's crust. Globally, acidic soil occupies 30-40% of ice-free land areas; Al toxicity is a major threat to crops. The first symptom of Al toxicity is the inhibition of root growth followed by poor root hair development, swollen root apices, necrosis of leaves and reduced yield. Although Rice (Oryza sativa) is an Al toxicity tolerant crop, it shows considerable variations among rice genotypes to Al exposure. Therefore, it is pertinent to understand Al toxicity and underlying mechanisms for Al tolerance in Rice. In the present study, 63 rice genotypes screened under Al stress showed significant variations of root growth. Expression stability of endogenous control genes (ECGs) revealed sulphite reductase (SR) as the most stable ECG that can be used as a reference gene for quantitative real-time PCR (qRT-PCR). Expression patterns of Al-responsive genes suggest genes associated with cytoskeletal dynamics, metabolism, and ion transporter could play significant roles in Al adaptation and tolerance in rice. The results showed Motodhan, Vietnam-1, Yimyu and N-861 as Al-toxicity tolerant, while Lespah, RCPL-13, VL-31329, and UPR2919-141-1 as most Al-sensitive genotypes among the studied rice lines cultivated in North-East India.
铝（Al）是地壳中含量第三丰富的元素。全球范围内，酸性土壤占无冰土地面积的 30-40%；铝毒性是作物面临的主要威胁之一。铝毒性的第一个症状是根系生长受到抑制，随后是根毛发育不良、根尖肿胀、叶片坏死和产量降低。尽管水稻（Oryza sativa）是一种耐铝毒性的作物，但它在不同水稻基因型中对铝暴露表现出相当大的差异。因此，了解水稻中的铝毒性及其耐铝机制至关重要。在本研究中，在铝胁迫下筛选的 63 个水稻基因型显示出根生长的显著变化。内参基因（ECGs）的表达稳定性表明亚硫酸盐还原酶（SR）是最稳定的 ECG，可作为定量实时 PCR（qRT-PCR）的参考基因。铝应答基因的表达模式表明，与细胞骨架动态、代谢和离子转运相关的基因可能在水稻的铝适应和耐受中发挥重要作用。结果表明，Motodhan、越南-1、Yimyu 和 N-861 对铝毒性具有耐受性，而 Lespah、RCPL-13、VL-31329 和 UPR2919-141-1 是在印度东北部种植的研究水稻品种中最敏感的铝基因型。
