多组学数据泛癌症分析显示 AAMP 是一个不利的预后标志物。
Pan-cancer analysis from multi-omics data reveals AAMP as an unfavourable prognostic marker.
机构信息
Department of General Surgical Department, Beijing Ditan Hospital, Capital Medical University, No. 8 Jing Shun East Street, Chaoyang District, Beijing, 100015, People's Republic of China.
Department of Pathology, Beijing Ditan Hospital, Capital Medical University, No. 8 Jing Shun East Street, Chaoyang District, Beijing, 100015, People's Republic of China.
出版信息
Eur J Med Res. 2023 Jul 27;28(1):258. doi: 10.1186/s40001-023-01234-z.
OBJECTIVES
Angio-associated migratory cell protein (AAMP) is a protein that participates in cell migration and is reported to be involved in cancer progression. However, the molecular mechanism of AAMP in pan-cancer is not known.
METHODS
We used multi-omics data, such as TIMER, TCGA, GTEx, CPTAC, HPA, and cBioPortal to analyze AAMP expression, and gene alteration in pan-cancer. Univariate Cox regression and Kaplan-Meier were utilized to explore prognostic significance of AAMP expression level. We applied Spearman analysis to investigate the correlation between AAMP and TMB, MSI, immune cell infiltration, immune checkpoints. Moreover, we mainly studied liver hepatocellular carcinoma(LIHC) to explore AAMP expression, clinical significance, and prognosis. Cox regression analysis was used to study independent factor to predict prognosis for AAMP in LIHC. GSEA was utilized to investigate the biological function for AAMP in LIHC.
RESULTS
AAMP was overexpressed in most cancers, and high AAMP expression was associated with worse overall survival (OS), disease-specific survival (DSS), and progress-free interval (PFI) for LIHC and adrenocortical carcinoma (ACC). Moreover, AAMP had the highest mutation frequency in uterine corpus endometrial carcinoma (UCEC). AAMP was correlated with TMB and MSI in esophageal carcinoma (ESCA), stomach adenocarcinoma (STAD), lung squamous cell carcinoma (LUSC), and thyroid carcinoma (THCA). Then, we focus on LIHC to investigate the expression and prognosis of AAMP. AAMP overexpression was related to histological grade and pathological stage in LIHC. Multivariate Cox regression analysis revealed that AAMP overexpression was an independent adverse prognostic marker for LIHC. AAMP expression was correlated with immune cell infiltration and immune checkpoints in LIHC. Function enrichment analysis indicated the participation of AAMP in the cell cycle and DNA replication.
CONCLUSIONS
AAMP was a latent prognostic indicator for pan-cancer and had high potential as a biomarker for the diagnosis and prognosis of LIHC.
目的
血管相关迁移细胞蛋白（AAMP）是一种参与细胞迁移的蛋白，据报道与癌症进展有关。然而，AAMP 在泛癌症中的分子机制尚不清楚。
方法
我们使用多组学数据，如 TIMER、TCGA、GTEx、CPTAC、HPA 和 cBioPortal 来分析泛癌症中的 AAMP 表达和基因改变。单变量 Cox 回归和 Kaplan-Meier 用于探索 AAMP 表达水平的预后意义。我们应用 Spearman 分析来研究 AAMP 与 TMB、MSI、免疫细胞浸润、免疫检查点的相关性。此外，我们主要研究了肝癌（LIHC）以探讨 AAMP 的表达、临床意义和预后。Cox 回归分析用于研究 AAMP 在 LIHC 中预测预后的独立因素。GSEA 用于研究 AAMP 在 LIHC 中的生物学功能。
结果
AAMP 在大多数癌症中过度表达，并且 AAMP 高表达与 LIHC 和肾上腺皮质癌（ACC）的总生存期（OS）、疾病特异性生存期（DSS）和无进展生存期（PFI）更差相关。此外，AAMP 在子宫体子宫内膜癌（UCEC）中的突变频率最高。AAMP 与食管癌（ESCA）、胃腺癌（STAD）、肺鳞状细胞癌（LUSC）和甲状腺癌（THCA）中的 TMB 和 MSI 相关。然后，我们专注于 LIHC 来研究 AAMP 的表达和预后。AAMP 过表达与 LIHC 的组织学分级和病理分期有关。多变量 Cox 回归分析表明，AAMP 过表达是 LIHC 的独立不良预后标志物。AAMP 表达与 LIHC 中的免疫细胞浸润和免疫检查点相关。功能富集分析表明 AAMP 参与了细胞周期和 DNA 复制。
结论
AAMP 是泛癌症的潜在预后指标，具有作为 LIHC 诊断和预后的生物标志物的高潜力。
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