Wako Y, Suzuki K, Goto Y, Kimura S
Tohoku J Exp Med. 1986 Jun;149(2):133-43. doi: 10.1620/tjem.149.133.
The effects of diabetes on the vitamin A metabolism were studied. The concentrations of plasma retinol and retinyl ester were measured in diabetic patients using high-performance liquid chromatography (HPLC). In diabetic patients, the mean level of retinyl ester was significantly elevated compared to that of normal subjects (diabetes, 68.3 +/- 42.5 IU/100 ml of plasma; study I, 87.6 +/- 64.3 IU/100 ml of plasma; study II vs. normal, 29.8 +/- 10.3 IU/100 ml of plasma). The ultracentrifugal and column chromatographic studies were carried out to examine the distribution of retinyl ester in patient's plasma. Ultracentrifugation study showed that the mean of 72.6% of retinyl ester was recovered in the lipoproteins of density higher than 1.006 and the remainder was recovered in the chylomicron (d less than 1.006). Gel filtration profiles for the separation of plasma vitamin A demonstrated that retinyl ester presented in the HDL, LDL and larger molecular size lipoprotein (suspected to be VLDL) as well as in the chylomicron.
研究了糖尿病对维生素A代谢的影响。使用高效液相色谱法（HPLC）测定糖尿病患者血浆视黄醇和视黄酯的浓度。与正常受试者相比，糖尿病患者视黄酯的平均水平显著升高（糖尿病组，68.3±42.5 IU/100 ml血浆；研究I，87.6±64.3 IU/100 ml血浆；研究II与正常组相比，29.8±10.3 IU/100 ml血浆）。进行超速离心和柱色谱研究以检查视黄酯在患者血浆中的分布。超速离心研究表明，平均72.6%的视黄酯在密度高于1.006的脂蛋白中回收，其余在乳糜微粒中回收（密度小于1.006）。用于分离血浆维生素A的凝胶过滤图谱表明，视黄酯存在于高密度脂蛋白（HDL）、低密度脂蛋白（LDL）和较大分子大小的脂蛋白（疑似极低密度脂蛋白（VLDL））以及乳糜微粒中。
