Almammadov Toghrul, Dirak Musa, Saymaz Ayca, Acari Alperen, Kolemen Safacan
Department of Chemistry, University of Zurich, Winterthurerstrasse 190, Zurich 8057, Switzerland.
Department of Chemistry, Koç University, Rumelifeneri Yolu, Istanbul 34450, Turkey.
Chem Commun (Camb). 2023 Aug 15;59(66):9972-9975. doi: 10.1039/d3cc02676k.
A resorufin-based dual-locked fluorescent probe (RHT) was introduced to image melanoma cells selectively. RHT was shown to function as an AND molecular logic gate as it emitted a signal only in the presence of both hydrogen sulfide (HS) and tyrosinase (Tyr), which are known to be overexpressed in melanoma cells. cell culture studies revealed that RHT can be activated with endogenous HS and Tyr and allows selective imaging of B16-F10 cancer cells under confocal microscopy. RHT marks the first ever example of a fluorescent probe that is sequentially activated by HS and Tyr.
一种基于试卤灵的双锁荧光探针（RHT）被用于选择性地对黑色素瘤细胞进行成像。RHT被证明可作为与门分子逻辑门，因为它仅在硫化氢（HS）和酪氨酸酶（Tyr）同时存在时才发出信号，而这两种物质在黑色素瘤细胞中已知会过度表达。细胞培养研究表明，RHT可被内源性HS和Tyr激活，并能在共聚焦显微镜下对B16-F10癌细胞进行选择性成像。RHT是首个由HS和Tyr依次激活的荧光探针实例。
