State Key Laboratory of Industrial Control Technology, Research Center for Analytical Instrumentation, Institute of Cyber-Systems and Control, College of Control Science and Engineering, Zhejiang University, Hangzhou 310000, China.
Biosensors (Basel). 2023 Jul 6;13(7):712. doi: 10.3390/bios13070712.
Spatial profiling technologies fill the gap left by the loss of spatial information in traditional single-cell sequencing, showing great application prospects. After just a few years of quick development, spatial profiling technologies have made great progress in resolution and simplicity. This review introduces the development of spatial omics sequencing based on microfluidic array chips and describes barcoding strategies using various microfluidic designs with simplicity and efficiency. At the same time, the pros and cons of each strategy are compared. Moreover, commercialized solutions for spatial profiling are also introduced. In the end, the future perspective of spatial omics sequencing and research directions are discussed.
空间分析技术填补了传统单细胞测序中空间信息丢失的空白，具有广阔的应用前景。在短短几年的快速发展之后，空间分析技术在分辨率和简易性方面取得了巨大的进步。本文综述了基于微流控芯片的空间组学测序技术的发展，并描述了利用各种微流控设计的简单高效的条码化策略。同时，比较了每种策略的优缺点。此外，还介绍了空间分析的商业化解决方案。最后，讨论了空间组学测序的未来前景和研究方向。
