Stanicka Karolina, Dobrucka Renata, Woźniak Magdalena, Sip Anna, Majka Jerzy, Kozak Wojciech, Ratajczak Izabela
Department of Chemistry, Faculty of Forestry and Wood Technology, Poznan University of Life Sciences, Wojska Polskiego 75, 60625 Poznań, Poland.
Department of Industrial Products and Packaging Quality, Institute of Quality Science, Poznań University of Economics and Business, al. Niepodległości 10, 61875 Poznań, Poland.
Polymers (Basel). 2021 Nov 10;13(22):3888. doi: 10.3390/polym13223888.
The aim of the research was to determine the influence of chitosan type and propolis extract concentration on biological and physicochemical properties of chitosan-propolis films in terms of their applicability in food packaging. The films were prepared using three types of chitosan: from crab shells, medium and high molecular weight and propolis concentration in the range of 0.75-5.0%. The prepared polysaccharide films were tested for antimicrobial properties, oxygen transmission rate (OTR) and water vapor transmission rate (WVTR). Moreover, sorption tests and structural analysis were carried out. Microbiological tests indicated the best antimicrobial activity for the film consisting of high molecular weight chitosan and 5.0% propolis extract. Both the type of chitosan and propolis concentration affected transmission parameters-OTR and WVTR. The best barrier properties were recorded for the film composed of high molecular weight chitosan and 5.0% propolis extract. The results of sorption experiments showed a slight influence of chitosan type and a significant effect of propolis extract concentration on equilibrium moisture content of tested films. Moreover, propolis extract concentration affected monolayer water capacity (Mm) estimated using the Guggenheim, Anderson and de Boer (GAB) sorption model. The obtained results indicate that chitosan films with an addition of propolis extract are promising materials for food packaging applications, including food containing probiotic microorganisms.
该研究的目的是从壳聚糖-蜂胶膜在食品包装中的适用性角度，确定壳聚糖类型和蜂胶提取物浓度对其生物学和物理化学性质的影响。使用三种类型的壳聚糖制备薄膜：来自蟹壳的中、高分子量壳聚糖以及浓度范围为0.75-5.0%的蜂胶。对制备的多糖薄膜进行了抗菌性能、氧气透过率（OTR）和水蒸气透过率（WVTR）测试。此外，还进行了吸附试验和结构分析。微生物学测试表明，由高分子量壳聚糖和5.0%蜂胶提取物组成的薄膜具有最佳抗菌活性。壳聚糖类型和蜂胶浓度均影响传输参数——OTR和WVTR。由高分子量壳聚糖和5.0%蜂胶提取物组成的薄膜具有最佳阻隔性能。吸附实验结果表明，壳聚糖类型对测试薄膜的平衡水分含量影响较小，而蜂胶提取物浓度影响显著。此外，蜂胶提取物浓度影响使用 Guggenheim、Anderson和de Boer（GAB）吸附模型估算的单层水容量（Mm）。所得结果表明，添加蜂胶提取物的壳聚糖薄膜是用于食品包装应用的有前景的材料，包括含有益生菌微生物的食品。
