Laboratory of Applied and Environmental Chemistry (LCAE), Faculty of Sciences, Mohammed First University, B.P. 717, Oujda 60000, Morocco.
Laboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Faculty of Sciences, Mohammed First University, B.P. 717, Oujda 60000, Morocco.
Mar Drugs. 2023 Jun 24;21(7):372. doi: 10.3390/md21070372.
In this study, a comparison was made of the chemical makeup of different extracts obtained from , a type of red seaweed that was gathered from the Nador lagoon situated in the northern part of Morocco. Additionally, their anti-diabetic and antioxidant properties were investigated. The application of GC-MS technology to analyze the fatty acid content of the samples revealed that linoleic acid and eicosenoic acid were the most abundant unsaturated fatty acids across all samples, with palmitic acid and oleic acid following in frequency. The HPLC analysis indicated that ascorbic and kojic acids were the most prevalent phenolic compounds, while apigenin was the most common flavonoid molecule. The aqueous extract exhibited significant levels of polyphenols and flavonoids, registering values of 381.31 ± 0.33 mg GAE/g and 201.80 ± 0.21 mg QE/g, respectively. Furthermore, this particular extract demonstrated a remarkable ability to scavenge DPPH radicals, as evidenced by its IC value of 0.17 ± 0.67 mg/mL. In addition, the methanolic extract was found to possess antioxidant properties, as evidenced by its ability to prevent β-carotene discoloration, with an IC ranging from 0.062 ± 0.02 mg/mL to 0.070 ± 0.06 mg/mL. In vitro study showed that all extracts significantly inhibited the enzymatic activity of α-amylase and α-glucosidase. Finally, molecular docking models were applied to assess the interaction between the primary phytochemicals identified in extracts and the human pancreatic α-amylase and α-glucosidase enzymes. The findings suggest that these extracts contain bioactive substances capable of reducing enzyme activity more effectively than the commercially available drug acarbose.
在这项研究中，比较了从红海中的一种海藻中提取的不同提取物的化学成分，这种海藻是从摩洛哥北部的纳多尔泻湖采集的。此外，还研究了它们的抗糖尿病和抗氧化特性。GC-MS 技术分析样品中的脂肪酸含量表明，亚油酸和二十碳烯酸是所有样品中最丰富的不饱和脂肪酸，其次是棕榈酸和油酸。HPLC 分析表明，抗坏血酸和曲酸是最常见的酚类化合物，而芹菜素是最常见的类黄酮分子。水提物表现出较高水平的多酚和类黄酮，分别达到 381.31±0.33mgGAE/g 和 201.80±0.21mgQE/g。此外，该提取物还具有显著的清除 DPPH 自由基的能力，其 IC 值为 0.17±0.67mg/mL。此外，甲醇提取物具有抗氧化特性，能够防止 β-胡萝卜素褪色，IC 范围为 0.062±0.02mg/mL 至 0.070±0.06mg/mL。体外研究表明，所有提取物均能显著抑制α-淀粉酶和α-葡萄糖苷酶的酶活性。最后，应用分子对接模型评估了从 提取物中鉴定出的主要植物化学物质与人类胰腺α-淀粉酶和α-葡萄糖苷酶之间的相互作用。研究结果表明，这些提取物含有生物活性物质，能够比市售药物阿卡波糖更有效地降低酶活性。
