Chen Zhigang, Xia Yongzhen, Liu Huaiwei, Liu Honglei, Xun Luying
State Key Laboratory of Microbial Technology, Shandong University, 72 Binhai Road, Qingdao 266237, China.
School of Molecular Biosciences, Washington State University, Pullman, WA 991647520, USA.
Antioxidants (Basel). 2021 Apr 22;10(5):646. doi: 10.3390/antiox10050646.
Elemental sulfur and sulfite have been used to inhibit the growth of yeasts, but thiosulfate has not been reported to be toxic to yeasts. We observed that thiosulfate was more inhibitory than sulfite to growing in a common yeast medium. At pH < 4, thiosulfate was a source of elemental sulfur and sulfurous acid, and both were highly toxic to the yeast. At pH 6, thiosulfate directly inhibited the electron transport chain in yeast mitochondria, leading to reductions in oxygen consumption, mitochondrial membrane potential and cellular ATP. Although thiosulfate was converted to sulfite and HS by the mitochondrial rhodanese Rdl1, its toxicity was not due to HS as the -deletion mutant that produced significantly less HS was more sensitive to thiosulfate than the wild type. Evidence suggests that thiosulfate inhibits cytochrome c oxidase of the electron transport chain in yeast mitochondria. Thus, thiosulfate is a potential agent against yeasts.
元素硫和亚硫酸盐已被用于抑制酵母的生长，但硫代硫酸盐尚未被报道对酵母有毒。我们观察到，在普通酵母培养基中，硫代硫酸盐比亚硫酸盐对酵母生长的抑制作用更强。在pH < 4时，硫代硫酸盐是元素硫和亚硫酸的来源，两者对酵母都具有高毒性。在pH 6时，硫代硫酸盐直接抑制酵母线粒体中的电子传递链，导致氧气消耗、线粒体膜电位和细胞ATP减少。尽管硫代硫酸盐被线粒体硫氰酸酶Rdl1转化为亚硫酸盐和HS，但它的毒性并非由于HS，因为产生显著更少HS的-缺失突变体比野生型对硫代硫酸盐更敏感。有证据表明，硫代硫酸盐抑制酵母线粒体中电子传递链的细胞色素c氧化酶。因此，硫代硫酸盐是一种潜在的抗酵母剂。
