Casalino Gaia, Dinardo Francesca Rita, D'Amico Francesco, Bozzo Giancarlo, Bove Antonella, Camarda Antonio, Lombardi Roberto, Dimuccio Michela Maria, Circella Elena
Department of Veterinary Medicine, University of Bari "Aldo Moro", S. P. Casamassima km 3, 70010 Valenzano, Italy.
Animals (Basel). 2023 Aug 16;13(16):2639. doi: 10.3390/ani13162639.
Colibacillosis, caused by , is responsible for economic losses in the poultry industry due to mortality, decreased production, and the cost of antibiotic treatments. Prevention of colibacillosis is based on improved biosecurity measures and the use of the vaccine performed with O78 strains, which is responsible for most cases of colibacillosis. Recently, there has been increased interest in other infection control methods, such as the use of natural compounds. The aim of this study was to evaluate the antimicrobial efficacy of cinnamon essential oil (CEO) against strains isolated from poultry. The MIC and MIC of CEO were determined by testing 117 strains belonging to serogroups O78, O2, O128, O139, isolated from laying hens (91 strains), broilers (10 strains), and turkeys (16 strains). The bacterial strains were tested at cell densities of 10 and 10 CFU/mL. At the cell density of 10 CFU/mL, MIC and MIC were 0.4 and 0.5 µL/mL for most of the tested strains, while they corresponded to 0.5 µL/mL for all strains isolated from broilers and for strains belonging to serogroup O139. At the cell density of 10 CFU/mL, MIC and MIC were 0.3 and 0.4 µL/mL, regardless of bird species of origin and for strains belonging to serogroups O78 and O2. In addition, a concentration of 0.04 µL/mL of CEO corresponded both to MIC and MIC for strains belonging to serogroups O139 and O128. Based on these results, cinnamon essential oil showed an effective antibacterial activity against strains from poultry and could find field application for the prevention of colibacillosis.
由大肠杆菌引起的大肠杆菌病，因死亡率、产量下降以及抗生素治疗成本，给家禽业造成经济损失。大肠杆菌病的预防基于改进生物安全措施以及使用针对O78菌株的疫苗，O78菌株是导致大多数大肠杆菌病病例的原因。最近，人们对其他感染控制方法的兴趣有所增加，例如使用天然化合物。本研究的目的是评估肉桂精油（CEO）对从家禽中分离出的大肠杆菌菌株的抗菌效果。通过测试从蛋鸡（91株）、肉鸡（10株）和火鸡（16株）中分离出的属于血清型O78、O2、O128、O139的117株菌株，确定了CEO的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。细菌菌株在细胞密度为10⁵和10⁶CFU/mL时进行测试。在细胞密度为10⁵CFU/mL时，大多数测试菌株的MIC和MBC分别为0.4和0.5µL/mL，而从肉鸡中分离出的所有菌株以及属于血清型O139的菌株的MIC和MBC均为0.5µL/mL。在细胞密度为10⁶CFU/mL时，无论菌株的来源禽类种类如何，以及属于血清型O78和O2的菌株，MIC和MBC分别为0.3和0.4µL/mL。此外，对于属于血清型O139和O128的菌株，0.04µL/mL的CEO浓度既对应MIC也对应MBC。基于这些结果，肉桂精油对家禽源大肠杆菌菌株显示出有效的抗菌活性，并可在预防大肠杆菌病方面找到实际应用。
