Gryaznova Mariya, Dvoretskaya Yulia, Burakova Inna, Syromyatnikov Mikhail, Popov Evgeny, Kokina Anastasia, Mikhaylov Evgeny, Popov Vasily
Laboratory of Metagenomics and Food Biotechnology, Voronezh State University of Engineering Technologies, 394036 Voronezh, Russia.
Department of Genetics, Cytology and Bioengineering, Voronezh State University, 394018 Voronezh, Russia.
Microorganisms. 2022 May 12;10(5):1020. doi: 10.3390/microorganisms10051020.
Probiotics are living microorganisms that provide numerous health benefits for their host. Probiotics have various effects on the body; for example, they change gut microbiota, improve the integrity of the epithelial barrier and have anti-inflammatory effects. The use of probiotic supplements that are based on lactic acid bacteria and bifidobacteria is one of the approaches that are used to balance gut microflora. In our study, we evaluated the effects of supplements, which were based on members of the family and bifidobacteria, on the gut microbiome of healthy mice using the sequencing method. The data that were obtained demonstrated that when mice received the probiotic supplements, statistically significant changes occurred in the composition of the microbiome at the phylum level, which were characterized by an increase in the number of , , and , all of which have potentially positive effects on health. At the generic level, a decrease in the abundance of members of the , and genus, which are involved in inflammatory processes, was observed for the group of mice that was fed with lactic acid bacteria. For the group of mice that was fed with bifidobacteria, a decrease was seen in the number of members of the and genus. The results of our study contribute to the understanding of changes in the gut microbiota of healthy mice under the influence of probiotics. It was shown that probiotics that are based on members of the family have a more positive effect on the gut microbiome than probiotics that are based on bifidobacteria.
益生菌是一类活的微生物，能为其宿主带来诸多健康益处。益生菌对身体有多种作用；例如，它们可改变肠道微生物群，改善上皮屏障的完整性并具有抗炎作用。使用基于乳酸菌和双歧杆菌的益生菌补充剂是用于平衡肠道微生物群的方法之一。在我们的研究中，我们使用测序方法评估了基于该菌属成员和双歧杆菌的补充剂对健康小鼠肠道微生物组的影响。所获得的数据表明，当小鼠接受益生菌补充剂时，微生物组在门水平的组成发生了具有统计学意义的变化，其特征是、、和的数量增加，所有这些对健康都可能具有积极影响。在属水平上，对于喂食乳酸菌的小鼠组，观察到参与炎症过程的属、属和属成员的丰度降低。对于喂食双歧杆菌的小鼠组，属和属的成员数量减少。我们的研究结果有助于理解益生菌影响下健康小鼠肠道微生物群的变化。结果表明，基于该菌属成员的益生菌对肠道微生物组的影响比基于双歧杆菌的益生菌更积极。
