Marclay Margaux, Dwyer Elizabeth, Suchodolski Jan S, Lidbury Jonathan A, Steiner Joerg M, Gaschen Frederic P
Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA.
Medi-Vet SA Vétérinaire, 1007 Lausanne, Switzerland.
Vet Sci. 2022 Jun 27;9(7):324. doi: 10.3390/vetsci9070324.
Antibiotics cause gut dysbiosis and bile acid dysmetabolism in dogs. The effect of fecal microbiota transplantation (FMT) on microbiome and metabolome recovery is unknown. This prospective, randomized, placebo-controlled study included sixteen healthy purpose-bred dogs. All dogs received tylosin 20 mg/kg PO once daily (days 1-7) and were randomly assigned to either receive one FMT via enema (day 8), daily oral FMT capsules (days 8-21), or daily placebo capsules (days 8-21). Fecal samples were frozen at regular intervals until day 42. Quantitative PCR for 8 bacterial taxa was performed to calculate the fecal dysbiosis index (FDI) and fecal concentrations of unconjugated bile acids (UBA) were measured using gas chromatography-mass spectrometry. Tylosin altered the abundance of most evaluated bacteria and induced a significant decrease in secondary bile acid concentrations at day 7 in all dogs. However, most parameters returned to their baseline by day 14 in all dogs. In conclusion, tylosin markedly impacted fecal microbiota and bile acid concentrations, although return to baseline values was quick after the antibiotic was discontinued. Overall, FMT did not accelerate recovery of measured parameters. Further studies are warranted to confirm the value of FMT in accelerating microbiota recovery in antibiotic-associated dysbiosis in dogs.
抗生素会导致犬类肠道菌群失调和胆汁酸代谢异常。粪便微生物群移植（FMT）对微生物组和代谢组恢复的影响尚不清楚。这项前瞻性、随机、安慰剂对照研究纳入了16只健康的纯种犬。所有犬只每天口服一次泰乐菌素20mg/kg（第1 - 7天），并随机分配接受一次灌肠FMT（第8天）、每日口服FMT胶囊（第8 - 21天）或每日口服安慰剂胶囊（第8 - 21天）。粪便样本定期冷冻至第42天。对8种细菌分类群进行定量PCR以计算粪便菌群失调指数（FDI），并使用气相色谱 - 质谱法测量未结合胆汁酸（UBA）的粪便浓度。泰乐菌素改变了大多数评估细菌的丰度，并在第7天导致所有犬只的次级胆汁酸浓度显著降低。然而，所有犬只的大多数参数在第14天恢复到基线水平。总之，泰乐菌素显著影响粪便微生物群和胆汁酸浓度，尽管在停用抗生素后恢复到基线值很快。总体而言，FMT并未加速所测参数的恢复。有必要进行进一步研究以证实FMT在加速犬类抗生素相关菌群失调中微生物群恢复方面的价值。
