Razola-Díaz María Del Carmen, Verardo Vito, Guerra-Hernández Eduardo Jesús, García-Villanova Ruiz Belén, Gómez-Caravaca Ana María
Department of Nutrition and Food Science, University of Granada, Campus of Cartuja, 18011 Granada, Spain.
Institute of Nutrition and Food Technology 'José Mataix', Biomedical Research Center, University of Granada, Avda. del Conocimiento s/n, 18100 Granada, Spain.
Antioxidants (Basel). 2023 Jul 11;12(7):1409. doi: 10.3390/antiox12071409.
Avocado peel and seed are the main by-products of avocado processing and are considered as promising sources of phenolic compounds with biological activities. Thus, this research focuses on the establishment, for the first time, of ultrasound-assisted extraction of flavan-3-ols with high antioxidant activity from avocado peel and seed using a sonotrode. Indeed, 2 Box-Behnken designs were performed for 15 experiments, with each design having three independent factors (ratio ethanol/water (/), time (min) and amplitude (%)). In both models, the responses included total procyanidins (flavan-3-ols) measured via HPLC-FLD and antioxidant activity measured via DPPH, ABTS and FRAP. The results showed that applying the sonotrode extraction method could increase flavan-3-ols recovery by 54% and antioxidant activity by 62-76% compared to ultrasound bath technology. Therefore, this technology was demonstrated to be a non-thermal, low time-consuming and scalable method that allowed the recovery of flavan-3-ols from avocado by-products that could be used as functional ingredients.
鳄梨果皮和果核是鳄梨加工的主要副产品，被认为是具有生物活性的酚类化合物的潜在来源。因此，本研究首次聚焦于使用超声探头从鳄梨果皮和果核中超声辅助提取具有高抗氧化活性的黄烷-3-醇。实际上，针对15次实验进行了2次Box-Behnken设计，每次设计有三个独立因素（乙醇/水比例（/）、时间（分钟）和振幅（%））。在这两个模型中，响应指标包括通过高效液相色谱-荧光检测法测定的总原花青素（黄烷-3-醇）以及通过DPPH、ABTS和FRAP测定的抗氧化活性。结果表明，与超声浴技术相比，应用超声探头提取方法可使黄烷-3-醇的回收率提高54%，抗氧化活性提高62%-76%。因此，该技术被证明是一种非热、耗时短且可扩展的方法，能够从鳄梨副产品中回收可作为功能成分的黄烷-3-醇。
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