Buitrago Ramírez Juan Rafael, Marreiro Gomes Robson Matheus, de Sousa Araujo Alan Carvalho, Muñoz Buitrago Sonia Astrid, Piraine Souza Jean, Monserrat José María
Programa de Pós Graduação em Aquicultura, Instituto de Oceanografia (IO), Universidade Federal do Rio Grande-FURG, Rua do Hotel, n° 2, Cassino, Rio Grande 96210-030, RS, Brazil.
Laboratório de Bioquímica Funcional de Organismos Aquáticos (BIFOA), Instituto de Oceanografia (IO), Universidade Federal do Rio Grande-FURG, Rua do Hotel, n° 2, Cassino, Rio Grande 96210-030, RS, Brazil.
Antioxidants (Basel). 2023 Jul 18;12(7):1439. doi: 10.3390/antiox12071439.
Lipoic acid (LA) is a mitochondrial coenzyme that, depending on the concentration and exposure time, can behave as an antioxidant or pro-oxidant agent and has a proven ability to modulate metabolism by promoting lipid and glucose oxidation for energy production. To assess the effects of LA on energy metabolism and redox balance over time, sp. nauplii was used as an animal model. The administered concentrations of the antioxidant were 0.05, 0.1, 0.5, 1.0, 5.0, and 10.0 µM. Therefore, possible differences in protein, triglyceride, glucose, and lactate concentrations in the artemia samples and total ammoniacal nitrogen (TAN) in the culture water were evaluated. We also measured the effects of LA on in vivo activity of the electron transport system (ETS), antioxidant capacity, and production of reactive oxygen species (ROS) at 6, 12, 18, and 24 h post-hatching. There was a decrease in glucose concentration in the LA-treated animals, and a decrease in ammonia production was observed in the 0.5 µM LA treatment. ETS activity was positively regulated by the addition of LA, with the most significant effects at concentrations of 5.0 and 10.0 µM at 12 and 24 h. For ETS activity, treatments with LA presented the highest values at 24 h, a period when ROS production decreased significantly, for the treatment with 10.0 µM. LA showed positive regulation of energy metabolism together with a decrease in ROS and TAN excretion.
硫辛酸（LA）是一种线粒体辅酶，根据其浓度和暴露时间，它可以作为抗氧化剂或促氧化剂，并且已被证明具有通过促进脂质和葡萄糖氧化以产生能量来调节新陈代谢的能力。为了评估LA随时间对能量代谢和氧化还原平衡的影响，卤虫无节幼体被用作动物模型。抗氧化剂的给药浓度为0.05、0.1、0.5、1.0、5.0和10.0μM。因此，评估了卤虫样品中蛋白质、甘油三酯、葡萄糖和乳酸浓度以及养殖水中总氨氮（TAN）的可能差异。我们还测量了LA在孵化后6、12、18和24小时对电子传递系统（ETS）的体内活性、抗氧化能力和活性氧（ROS）产生的影响。LA处理的动物体内葡萄糖浓度降低，在0.5μM LA处理中观察到氨产量下降。添加LA对ETS活性有正向调节作用，在12和24小时时，5.0和10.0μM浓度的影响最为显著。对于ETS活性，LA处理在24小时时呈现最高值，此时对于10.0μM的处理，ROS产生显著下降。LA显示出对能量代谢的正向调节作用，同时ROS和TAN排泄减少。
