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使用LP95发酵豆浆的抗氧化特性

Antioxidative Properties of Fermented Soymilk Using LP95.

作者信息

Letizia Francesco, Fratianni Alessandra, Cofelice Martina, Testa Bruno, Albanese Gianluca, Di Martino Catello, Panfili Gianfranco, Lopez Francesco, Iorizzo Massimo

机构信息

Department of Agriculture, Environmental and Food Sciences, University of Molise, Via De Sanctis, 86100 Campobasso, Italy.

出版信息

Antioxidants (Basel). 2023 Jul 18;12(7):1442. doi: 10.3390/antiox12071442.

Abstract

In recent times, there has been a growing consumer interest in replacing animal foods with alternative plant-based products. Starting from this assumption, for its functional properties, soymilk fermented with lactic acid bacteria is gaining an important position in the food industry. In the present study, soymilk was fermented with LP95 at 37 °C, without the use of stabilizers as well as thickeners and acidity regulators. We evaluated the antioxidant capacity of fermented soymilk along with its enrichment in aglycone isoflavones. The conversion of isoflavone glucosides to aglycones (genistein, glycitein, and daidzein) was analyzed together with antioxidant activity (ABTS) measurements, lipid peroxidation measurements obtained by a thiobarbituric acid reactive substance (TBARS) assay, and apparent viscosity measurements. From these investigations, soymilk fermentation using LP95 as a starter significantly increased isoflavones' transformation to their aglycone forms. The content of daidzein, glycitein, and genistein increased after 24 h of fermentation, reaching levels of 48.45 ± 1.30, 5.10 ± 0.16, and 56.35 ± 1.02 μmol/100 g of dry weight, respectively. Furthermore, the antioxidant activity increased after 6 h with a reduction in MDA (malondialdehyde). The apparent viscosity was found to increase after 24 h of fermentation, while it slightly decreased, starting from 21 days of storage. Based on this evidence, LP95 appears to be a promising candidate as a starter for fermented soymilk production.

摘要

近年来,消费者对用替代性植物基产品替代动物食品的兴趣日益浓厚。基于这一假设,由于其功能特性,用乳酸菌发酵的豆浆在食品工业中占据了重要地位。在本研究中,豆浆在37℃下用LP95进行发酵,未使用稳定剂、增稠剂和酸度调节剂。我们评估了发酵豆浆的抗氧化能力及其在苷元异黄酮方面的富集情况。分析了异黄酮糖苷向苷元(染料木黄酮、黄豆黄素和大豆苷元)的转化,并进行了抗氧化活性(ABTS)测量、通过硫代巴比妥酸反应物质(TBARS)测定法获得的脂质过氧化测量以及表观粘度测量。通过这些研究,以LP95作为发酵剂对豆浆进行发酵显著提高了异黄酮向其苷元形式的转化。发酵24小时后,大豆苷元、黄豆黄素和染料木黄酮的含量分别增加到48.45±1.30、5.10±0.16和56.35±1.02μmol/100克干重。此外,抗氧化活性在6小时后增加,同时丙二醛(MDA)减少。发酵24小时后表观粘度增加,而从储存21天开始略有下降。基于这些证据,LP95似乎是发酵豆浆生产发酵剂的一个有前途的候选者。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76a7/10376881/8d5c0354332a/antioxidants-12-01442-g001.jpg

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