Transcriptome-Based Identification of the Muscle Tissue-Specific Expression Gene and Its Regulation of Proliferation, Apoptosis and Differentiation in Chicken Primary Myoblasts.

Skeletal muscle is an essential tissue in meat-producing animals, and meat-producing traits have been a hot topic in chicken genetic breeding research. Current research shows that () is one of the most abundant proteins in skeletal muscle and plays an important role in the growth and development of skeletal muscle, but its role in the development of chicken skeletal muscle is still unclear. Via RNA sequencing (RNA-seq), we found that was highly expressed in chicken breast muscle tissue. In this study, the expression profile of was examined by quantitative real-time PCR (qPCR), and overexpression and RNA interference techniques were used to explore the functions of in the proliferation, apoptosis and differentiation of chicken primary myoblasts (CPMs). It was shown that was specifically highly expressed in breast muscle and leg muscle and was highly expressed in stage 16 embryonic muscle, while inhibited proliferation, promoted the apoptosis and differentiation of CPMs and was involved in regulating chicken myogenesis. Transcriptome sequencing was used to identify genes that were differentially expressed in CPMs after disruption, and bioinformatics analysis showed that was involved in regulating chicken myogenesis. In summary, plays an important role in skeletal muscle development during chicken growth and development.