Chiti Lavinia E, Park Brian, d'Orchymont Faustine, Holland Jason P, Nolff Mirja C
Klinik für Kleintierchirurgie, Vetsuisse-Fakultät, University of Zurich, Wintherturerstrasse 260, CH-8057 Zurich, Switzerland.
Department of Chemistry, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland.
Animals (Basel). 2023 Jul 20;13(14):2363. doi: 10.3390/ani13142363.
Fluorescence-guided surgery can aid in the intraoperative visualization of target tissues, with promising applications in human and veterinary surgical oncology. The aim of this study was to evaluate the performances of two fluoresce camera systems, IC-Flow and Visionsense VS3 Iridum, for the detection of two non-targeted (ICG and IRDye-800) and two targeted fluorophores (Angiostamp and FAP-Cyan) under different room light conditions, including ambient light, new generation LED, and halogen artificial light sources, which are commonly used in operating theaters. Six dilutions of the fluorophores were imaged in phantom kits using the two camera systems. The limit of detection (LOD) and mean signal-to-background ratio (mSBR) were determined. The highest values of mSBR and a lower LOD were obtained in dark conditions for both systems. Under room lights, the capabilities decreased, but the mSBR remained greater than 3 (=clearly detectable signal). LOD and mSBR worsened under surgical lights for both camera systems, with a greater impact from halogen bulbs on Visionsense VS3 Iridium and of the LED lights on IC-Flow due to a contribution of these lights in the near-infrared spectrum. When considering implementing FGS into the clinical routine, surgeons should cautiously evaluate the spectral contribution of the lights in the operating theater.
荧光引导手术有助于在术中可视化目标组织，在人类和兽医外科肿瘤学中具有广阔的应用前景。本研究的目的是评估两种荧光相机系统IC-Flow和Visionsense VS3 Iridum在不同室内光照条件下检测两种非靶向荧光团（吲哚菁绿和IRDye-800）和两种靶向荧光团（血管标记物和FAP-青色荧光团）的性能，这些光照条件包括手术室常用的环境光、新一代LED光和卤素人造光源。使用这两种相机系统对六种荧光团稀释液在模拟试剂盒中进行成像。确定了检测限（LOD）和平均信号背景比（mSBR）。在黑暗条件下，两种系统均获得了最高的mSBR值和较低的LOD。在室内光线下，性能下降，但mSBR仍大于3（=可清晰检测到的信号）。在手术灯光下，两种相机系统的LOD和mSBR均变差，由于这些光在近红外光谱中的贡献，卤素灯泡对Visionsense VS3 Iridium的影响更大，而LED灯对IC-Flow的影响更大。在考虑将荧光引导手术应用于临床常规时，外科医生应谨慎评估手术室灯光的光谱贡献。
