Ren Chunhuan, Sun Zhipeng, Chen Yale, Chen Jiahong, Wang Shijia, Liu Qingqing, Wang Penghui, Cheng Xiao, Zhang Zijun, Wang Qiangjun
College of Animal Science and Technology, Anhui Agriculture University, Hefei 230036, China.
New Rural Develop Research Institute, Anhui Agricultural University, Hefei 230036, China.
Animals (Basel). 2023 Jul 20;13(14):2368. doi: 10.3390/ani13142368.
Sperm proteins play vital roles in improving sperm freezing resilience in domestic animals. However, it remains poorly defined which proteins regulate the freezing resilience of spermatozoa in rams (). Here, we compared the proteome of ram sperm with a high cryopreservation recovery ratio (HCR) with that of ram sperm with a low cryopreservation recovery ratio (LCR) using a tandem mass tag-based quantitative proteomics approach. Bioinformatic analysis was performed to evaluate differentially expressed proteins (DEPs). A total of 2464 proteins were identified, and 184 DEPs were screened. Seventy-two proteins were higher in the LCR group. One hundred and twelve proteins were more abundant in the HCR group, and they were mainly involved in the regulation of oxidative phosphorylation and thermogenesis pathways. Proteins in high abundance in the HCR group included the S100A family, such as S100A8, S100A9, S100A14, and S100A16, effectively controlling for CA and maintaining flagella structure; HYOU1 and PRDX1, which participate in antioxidant protection and anti-apoptosis to prevent cell death; and HSP90B1, which maintains cell activity and immune response. Our results could help illuminate the molecular mechanisms underlying cryopreservation of ram semen and expand the potential direction of cryopreservation of high-quality semen.
精子蛋白在提高家畜精子冷冻恢复力方面发挥着至关重要的作用。然而，关于哪些蛋白质调节公羊精子的冷冻恢复力仍不清楚。在此，我们使用基于串联质量标签的定量蛋白质组学方法，比较了冷冻保存恢复率高（HCR）的公羊精子与冷冻保存恢复率低（LCR）的公羊精子的蛋白质组。进行了生物信息学分析以评估差异表达蛋白（DEP）。共鉴定出2464种蛋白质，筛选出184种DEP。72种蛋白质在LCR组中含量较高。112种蛋白质在HCR组中含量更丰富，它们主要参与氧化磷酸化和产热途径的调节。HCR组中高丰度的蛋白质包括S100A家族，如S100A8、S100A9、S100A14和S100A16，可有效控制钙离子并维持鞭毛结构；HYOU1和PRDX1，参与抗氧化保护和抗凋亡以防止细胞死亡；以及HSP90B1，维持细胞活性和免疫反应。我们的结果有助于阐明公羊精液冷冻保存的分子机制，并拓展高质量精液冷冻保存的潜在方向。
