Zhang Zhuohui, Li Jiyun, Zhou Rushun, Xu Qianqian, Qu Shiyin, Lin Hongguang, Wang Yan, Li Pishun, Zheng Xiaofeng
College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China.
Hunan Engineering Technology Research Center of Veterinary Drugs, Hunan Agricultural University, Changsha 410128, China.
Antibiotics (Basel). 2023 Jul 12;12(7):1178. doi: 10.3390/antibiotics12071178.
Non-typhoidal (NTS) is a foodborne pathogen and a prevalent causative agent for disease outbreaks globally. The enterica serovar 4,[5],12:i:- (S.4,[5],12:i:-) belongs to the monophasic variant of , which is of current global concern. In this study, the epidemiology and genomic characterization of S. 4,[5],12:i:- isolates from 17 livestock farms in Hunan Province between 2019 and 2020, as well as their susceptibility to 14 antimicrobial agents, were profiled. Twelve serotypes were identified using the White-Kauffmann-Le Minor scheme, and whole-genome sequencing analyses were conducted based on these isolates. Overall, 107 strains were isolated, of which 73% (78/107) were multidrug resistant. Resistance to tetracycline (85.05%) was found to be the most prevalent, followed by the and genes. (monophasic) 4,[5],12:i:- was the most common serotype, followed by and Most antimicrobial-resistant strains were isolated from pigs, indicating that they could be important reservoirs of resistant non-typhoidal strains. The presence of similar genetic environments in S. 4,[5],12:i:- indicates both vertical and horizontal transmission of resistance plasmids, which may promote the spread of drug resistance genes. Appropriate measures should be taken to curb the prevalence of S. 4,[5],12:i:-.
非伤寒沙门氏菌（NTS）是一种食源性病原体，也是全球疾病暴发的常见致病因子。肠炎血清型4,[5],12:i:-（鼠伤寒沙门氏菌4,[5],12:i:-）属于单相变体，是当前全球关注的对象。在本研究中，对2019年至2020年间从湖南省17个畜牧场分离出的鼠伤寒沙门氏菌4,[5],12:i:-菌株的流行病学、基因组特征及其对14种抗菌药物的敏感性进行了分析。使用怀特-考夫曼-勒米诺分型方案鉴定出12种血清型，并基于这些分离株进行了全基因组测序分析。总体而言，共分离出107株菌株,其中73%（78/107）为多重耐药菌株。发现对四环素的耐药率最高（85.05%），其次是[相关基因]。单相4,[5],12:i:-是最常见的血清型，其次是[其他血清型]。大多数耐药菌株分离自猪，这表明猪可能是耐药非伤寒沙门氏菌菌株的重要宿主。鼠伤寒沙门氏菌4,[5],12:i:-中相似遗传环境的存在表明耐药质粒的垂直和水平传播，这可能会促进耐药基因的传播。应采取适当措施遏制鼠伤寒沙门氏菌4,[5],12:i:-的流行。
