Luo Denglin, Li Xingguo, Geng Mengyuan, Zhang Yunhui, Lan Honglin, Li Jiale, Qi Caili, Bai Zhouya, Huang Jihong
Food and Pharmacy College, Xuchang University, Xuchang 461000, China.
Henan Engineering Research Center of Food Material, College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China.
Foods. 2023 Jul 8;12(14):2640. doi: 10.3390/foods12142640.
Arabinoxylan (AX) is a dietary fiber that has been proven to have a significant antidiabetic effect. Liver metabolic disorders frequently coincide with the development of type 2 diabetes, but research on the hepatoprotective effects of AX in type 2 diabetic mice is lacking. As AX is abundant in the wastewater produced during vital wheat gluten protein production, this study used it as a raw material to evaluate its protective effect on liver function. The study employed an AX intervention in type 2 diabetic mice induced by a high-fat diet combined with streptozotocin and collected serum and liver tissue samples after 4 weeks. Serum and liver function indicators were measured using an automatic biochemistry analysis apparatus, and liver fat accumulation was observed using oil red O staining. Nontargeted metabolomics analysis of liver tissues was conducted using UHPLC-MS/MS. The results showed that AX significantly improved liver function indicators and histopathological damage, and regulated liver metabolic disorders by improving the differential metabolites of pantothenate and CoA biosynthesis, as well as purine metabolism. This study demonstrated that AX may exert a significant hepatoprotective effect by regulating metabolic disorders.
阿拉伯木聚糖（AX）是一种膳食纤维，已被证明具有显著的抗糖尿病作用。肝脏代谢紊乱常与2型糖尿病的发展同时出现，但关于AX对2型糖尿病小鼠肝脏保护作用的研究尚缺。由于AX在谷朊粉蛋白生产过程中产生的废水中含量丰富，本研究以此为原料评估其对肝功能的保护作用。该研究对高脂饮食联合链脲佐菌素诱导的2型糖尿病小鼠进行AX干预，并在4周后采集血清和肝脏组织样本。使用自动生化分析仪测定血清和肝功能指标，用油红O染色观察肝脏脂肪蓄积情况。采用超高效液相色谱-串联质谱法对肝脏组织进行非靶向代谢组学分析。结果表明，AX显著改善肝功能指标和组织病理学损伤，并通过改善泛酸和辅酶A生物合成以及嘌呤代谢的差异代谢物来调节肝脏代谢紊乱。本研究表明，AX可能通过调节代谢紊乱发挥显著的肝脏保护作用。
