Wang Na, Pei Haiwei, Xiang Wenzhou, Li Tao, Lin Shengjie, Wu Jiayi, Chen Zishuo, Wu Houbo, Li Chuanmao, Wu Hualian
CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, Institution of South China Sea Ecology and Environmental Engineering, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
Foods. 2023 Jul 10;12(14):2652. doi: 10.3390/foods12142652.
In order to rapidly screen microalgae species as feedstocks for antioxidants, extracts were obtained from 16 microalgae strains (under 11 genera, 7 classes) using two methods: a one-step extraction with ethanol/water and a three-step fractionating procedure using hexane, ethylacetate, and water successively. Measuring the total phenol content (TPC), total carotenoid content (TCC), and antioxidant activity of the extracts, indicating TPC and TCC, played an important role in determining the antioxidant activity of the microalgae. A weighted scoring system was used to evaluate the antioxidant activity, and the scores of microalgal samples from two extraction methods were calculated using the same system. Among the investigated microalgae, SCSIO-46781 had the highest antioxidant score, contributing to high TPC and TCC, followed by SCSIO-44012, sp. SCSIO-45224, SCSIO-45120, and sp. SCSIO-45006, respectively. Additionally, the above-mentioned five strains are currently being applied in commercial production, indicating this system could be effective not only for screening microalgal antioxidants, but also for screening microalgal species/strains with strong adaptation to environmental stress, which is a critical trait for their commercial cultivation.
为了快速筛选作为抗氧化剂原料的微藻物种，使用两种方法从16种微藻菌株（隶属于11个属、7个纲）中提取提取物：用乙醇/水一步提取法和依次使用己烷、乙酸乙酯和水的三步分级分离法。测量提取物的总酚含量（TPC）、总类胡萝卜素含量（TCC）和抗氧化活性，结果表明TPC和TCC在确定微藻的抗氧化活性中起重要作用。采用加权评分系统评估抗氧化活性，并使用相同系统计算两种提取方法的微藻样品得分。在所研究的微藻中，SCSIO - 46781的抗氧化得分最高，这归因于其高TPC和TCC，其次分别是SCSIO - 44012、SCSIO - 45224、SCSIO - 45120和SCSIO - 45006。此外，上述五个菌株目前正在商业生产中应用，这表明该系统不仅可有效筛选微藻抗氧化剂，还可用于筛选对环境胁迫具有强适应性的微藻物种/菌株，这是其商业养殖的关键特性。
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