Kasyanenko Nina, Baryshev Andrei, Artamonova Daria, Sokolov Petr
Faculty of Physics, Saint Petersburg State University, Saint Petersburg 199034, Russia.
Entropy (Basel). 2023 Jul 12;25(7):1052. doi: 10.3390/e25071052.
The transformation of high-molecular DNA from a random swollen coil in a solution to a discrete nanosized particle with the ordered packaging of a rigid and highly charged double-stranded molecule is one of the amazing phenomena of polymer physics. DNA condensation is a well-known phenomenon in biological systems, yet its molecular mechanism is not clear. Understanding the processes occurring in vivo is necessary for the usage of DNA in the fabrication of new biologically significant nanostructures. Entropy plays a very important role in DNA condensation. DNA conjugates with metal nanoparticles are useful in various fields of nanotechnology. In particular, they can serve as a basis for creating multicomponent nanoplatforms for theranostics. DNA must be in a compact state in such constructions. In this paper, we tested the methods of DNA integration with silver, gold and palladium nanoparticles and analyzed the properties of DNA conjugates with metal nanoparticles using the methods of atomic force microscopy, spectroscopy, viscometry and dynamic light scattering. DNA size, stability and rigidity (persistence length), as well as plasmon resonance peaks in the absorption spectra of systems were studied. The methods for DNA condensation with metal nanoparticles were analyzed.
高分子DNA在溶液中从随机的肿胀线圈转变为具有刚性且高度带电的双链分子有序包装的离散纳米级颗粒，是高分子物理学中令人惊奇的现象之一。DNA凝聚在生物系统中是一种众所周知的现象，但其分子机制尚不清楚。了解体内发生的过程对于在制造新的具有生物学意义的纳米结构中使用DNA是必要的。熵在DNA凝聚中起着非常重要的作用。DNA与金属纳米颗粒的缀合物在纳米技术的各个领域都很有用。特别是，它们可以作为创建用于治疗诊断的多组分纳米平台的基础。在这种结构中，DNA必须处于紧凑状态。在本文中，我们测试了DNA与银、金和钯纳米颗粒整合的方法，并使用原子力显微镜、光谱学、粘度测定和动态光散射方法分析了DNA与金属纳米颗粒缀合物的性质。研究了DNA的大小、稳定性和刚性（持久长度），以及系统吸收光谱中的等离子体共振峰。分析了用金属纳米颗粒使DNA凝聚的方法。
