Subtropical Livestock Research Institute, National Institute of Animal Science, RDA, Jeju 63242, Republic of Korea.
Planning and Coordination Division, National Institute of Animal Science, RDA, Wanju 55365, Republic of Korea.
Genes (Basel). 2023 Jun 27;14(7):1354. doi: 10.3390/genes14071354.
The influences of diet and environmental factors on gut microbial profiles have been widely acknowledged; however, the specific roles of host genetics remain uncertain. To unravel host genetic effects, we raised 47 Jeju crossbred (Jeju × Thoroughbred) foals that exhibited higher genetic diversity. Foals were raised under identical environmental conditions and diets. Microbial composition revealed that , , and were the predominant phyla. We identified 31 host-microbiome associations by utilizing 47,668 single nucleotide polymorphisms (SNPs) and 734 taxa with quantitative trait locus (QTL) information related to horse growth. The taxa involved in 31 host-microbiome associations were functionally linked to carbohydrate metabolism, energy metabolic processes, short-chain fatty acid (SCFA) production, and lactic acid production. Abundances of these taxa were affected by specific SNP genotypes. Most growth-associated SNPs are found between genes. The rs69057439 and rs69127732 SNPs are located within the introns of the and genes, respectively. These genes are known to affect energy balance and metabolism. These discoveries emphasize the significant effect of host SNPs on the development of the intestinal microbiome during the initial phases of life and provide insights into the influence of gut microbial composition on horse growth.
饮食和环境因素对肠道微生物群的影响已得到广泛认可；然而，宿主遗传的具体作用仍不确定。为了阐明宿主遗传效应，我们饲养了 47 头济州杂交（济州 × 纯血马）小马，它们表现出更高的遗传多样性。小马在相同的环境条件和饮食下饲养。微生物组成表明， 、 和 是主要的门。我们通过利用与马生长相关的 47668 个单核苷酸多态性（SNP）和 734 个具有数量性状基因座（QTL）信息的分类群，鉴定了 31 个宿主-微生物群关联。涉及 31 个宿主-微生物群关联的分类群与碳水化合物代谢、能量代谢过程、短链脂肪酸（SCFA）产生和乳酸产生功能相关。这些分类群的丰度受特定 SNP 基因型的影响。大多数与生长相关的 SNP 位于基因之间。rs69057439 和 rs69127732 SNP 分别位于 和 基因的内含子中。这些基因已知会影响能量平衡和代谢。这些发现强调了宿主 SNP 在生命早期肠道微生物群发育中的重要作用，并深入了解了肠道微生物组成对马生长的影响。
