Department of Plants, Soils, and Climate/Center for Integrated BioSystems, College of Agriculture and Applied Sciences, Utah State University, Logan, UT 84322, USA.
Bioinformatics Facility, Center for Integrated BioSystems, Utah State University, Logan, UT 84322, USA.
Genes (Basel). 2023 Jul 20;14(7):1481. doi: 10.3390/genes14071481.
Microsatellites, also known as simple sequence repeats (SSRs), are polymorphic loci that play an important role in genome research, animal breeding, and disease control. Ranch animals are important components of agricultural landscape. The ranch animal SSR database, , is a web resource which contains 15,520,263 putative SSR markers. This database provides a comprehensive tool for performing end-to-end marker selection, from SSRs prediction to generating marker primers and their cross-species feasibility, visualization of the resulting markers, and finding similarities between the genomic repeat sequences all in one place without the need to switch between other resources. The user-friendly online interface allows users to browse SSRs by genomic coordinates, repeat motif sequence, chromosome, motif type, motif frequency, and functional annotation. Users may enter their preferred flanking area around the repeat to retrieve the nucleotide sequence, they can investigate SSRs present in the genic or the genes between SSRs, they can generate custom primers, and they can also execute in silico validation of primers using electronic PCR. For customized sequences, an SSR prediction pipeline called is also built. New species will be added to this website's database on a regular basis throughout time. To improve animal health via genomic selection, we hope that will be a useful tool for mapping quantitative trait loci (QTLs) and marker-assisted selection. The web-resource is freely accessible at https://bioinfo.usu.edu/ranchSATdb/.
微卫星,也称为简单重复序列 (SSR),是多态性基因座,在基因组研究、动物育种和疾病控制中发挥着重要作用。牧场动物是农业景观的重要组成部分。牧场动物 SSR 数据库 ,是一个包含 15,520,263 个假定 SSR 标记的网络资源。该数据库为执行端到端的标记选择提供了一个全面的工具,从 SSR 预测到生成标记引物及其跨物种可行性、标记可视化以及在一个地方找到基因组重复序列之间的相似性,而无需在其他资源之间切换。用户友好的在线界面允许用户按基因组坐标、重复基序序列、染色体、基序类型、基序频率和功能注释浏览 SSR。用户可以输入他们喜欢的重复周围的侧翼区域来检索核苷酸序列,他们可以研究基因内或 SSR 之间的 SSR,他们可以生成自定义引物,还可以使用电子 PCR 对引物进行虚拟验证。对于定制序列,还构建了一个名为 的 SSR 预测管道。新的物种将定期添加到本网站的数据库中。为了通过基因组选择改善动物健康,我们希望 将成为图谱数量性状基因座 (QTL) 和标记辅助选择的有用工具。该网络资源可在 https://bioinfo.usu.edu/ranchSATdb/ 免费获取。