Academy of Animal Science and Veterinary Medicine, Qinghai University, Xining, China.
State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, China.
Vet Med Sci. 2021 Jul;7(4):1332-1338. doi: 10.1002/vms3.371. Epub 2021 May 6.
Simple sequence repeats (SSRs) are an important part of the genome and have become powerful auxiliary DNA markers in animal breeding using marker-assisted selection (MAS). Based on previous sequencing data of Qinghai Bamei pigs, a total of three novel candidate SSR loci were analysed in this study. Time-of-flight mass spectrometry (TOF-MS) was used for SSR genotyping, and association analyses between SSRs and the litter size of Qinghai Bamei sows was also performed. The results of genotyping showed that the (ATC) -P1, (AC) -P2 and (AC) -P3 loci had 2, 3 and 18 genotypes, respectively; 2, 3 and 8 alleles were also identified at these loci. Except for the (AC) -P2 locus, the polymorphism information content (PIC) values of other loci were greater than 0.25. Association analyses indicated that only the (ATC) -P1 locus was significantly associated with the litter size of Qinghai Bamei sows (p = .047). Compared to 189-/189- genotype, individuals with the 189-/195- genotype had the senior litter size, which was 9.04 ± 0.21. Our results enrich the data on SSRs in Qinghai Bamei pigs and indicate that (ATC) -P1 is a candidate locus for MAS in the pig industry.
简单序列重复（SSR）是基因组的重要组成部分，已成为动物育种中使用标记辅助选择（MAS）的有力辅助 DNA 标记。本研究基于青海八眉猪的测序数据，分析了三个新的候选 SSR 位点。飞行时间质谱（TOF-MS）用于 SSR 基因分型，还进行了 SSR 与青海八眉猪窝产仔数的关联分析。基因分型结果表明，（ATC）-P1、（AC）-P2 和（AC）-P3 位点分别有 2、3 和 18 种基因型；在这些位点也鉴定到 2、3 和 8 个等位基因。除（AC）-P2 位点外，其他位点的多态信息含量（PIC）值均大于 0.25。关联分析表明，只有（ATC）-P1 位点与青海八眉猪窝产仔数显著相关（p=0.047）。与 189-/189-基因型相比，189-/195-基因型个体的窝产仔数较高，为 9.04±0.21。本研究结果丰富了青海八眉猪 SSR 数据，并表明（ATC）-P1 是猪产业 MAS 的候选位点。
