Ambros Stefanie, Kotewitsch Mona, Wittig Philipp R, Bammer Bettina, Mustroph Angelika
Department of Plant Physiology, University of Bayreuth, Bayreuth, Germany.
Front Plant Sci. 2022 Apr 29;13:897673. doi: 10.3389/fpls.2022.897673. eCollection 2022.
Waterlogging is one major stress for crops and causes multiple problems for plants, for example low gas diffusion, changes in redox potential and accumulation of toxic metabolites. is an important oil crop with high waterlogging sensitivity, which may cause severe yield losses. Its reactions to the stress are not fully understood. In this work the transcriptional response of rapeseed to one aspect of waterlogging, hypoxia in the root zone, was analyzed by RNAseq, including two rapeseed cultivars from different origin, Avatar from Europe and Zhongshuang 9 from Asia. Both cultivars showed a high number of differentially expressed genes in roots after 4 and 24 h of hypoxia. The response included many well-known hypoxia-induced genes such as genes coding for glycolytic and fermentative enzymes, and strongly resembled the hypoxia response of the model organism . The carbohydrate status of roots, however, was minimally affected by root hypoxia, with a tendency of carbohydrate accumulation rather than a carbon starvation. Leaves did not respond to the root stress after a 24-h treatment. In agreement with the gene expression data, subsequent experiments with soil waterlogging for up to 14 days revealed no differences in response or tolerance to waterlogging between the two genotypes used in this study. Interestingly, using a 0.1% starch solution for waterlogging, which caused a lowered soil redox potential, resulted in much stronger effects of the stress treatment than using pure water suggesting a new screening method for rapeseed cultivars in future experiments.
涝害是作物面临的主要胁迫之一，会给植物带来多种问题，例如气体扩散率低、氧化还原电位变化以及有毒代谢物积累。油菜是一种对涝害敏感的重要油料作物，涝害可能导致严重的产量损失。人们对其在这种胁迫下的反应尚未完全了解。在这项研究中，通过RNA测序分析了油菜对涝害一个方面即根区缺氧的转录反应，研究材料包括两个来自不同产地的油菜品种，欧洲的Avatar和亚洲的中双9号。在缺氧处理4小时和24小时后，两个品种的根部均显示出大量差异表达基因。这种反应包括许多众所周知的缺氧诱导基因，如编码糖酵解和发酵酶的基因，并且与模式生物的缺氧反应非常相似。然而，根部的碳水化合物状态受根区缺氧的影响极小，呈现出碳水化合物积累的趋势而非碳饥饿状态。经过24小时处理后，叶片对根部胁迫没有反应。与基因表达数据一致，随后进行的长达14天的土壤淹水实验表明，本研究中使用的两个基因型在对涝害的反应或耐受性方面没有差异。有趣的是，使用0.1%淀粉溶液进行淹水处理，这会导致土壤氧化还原电位降低，与使用纯水相比，胁迫处理产生的影响要强得多，这表明在未来的实验中可以采用一种新的油菜品种筛选方法。
