Laboratory of Materials and Interfacial Systems, Faculty of Science, Abdelmalek Essaadi University, BP 2121, Tetouan 93002, Morocco.
Research Group of Advanced Materials, Structures and Civil Engineering, National School of Applied Sciences of Tetouan, Abdelmalek Essaadi University, BP 2121, Tetouan 93002, Morocco.
Int J Mol Sci. 2023 Jul 10;24(14):11269. doi: 10.3390/ijms241411269.
Paracetamol (PCT), or acetaminophen, is an important drug used worldwide for various clinical purposes. However, the excessive or indiscriminate use of PCT can provoke liver and kidney dysfunction; hence, it is essential to determine the amount of this target in biological samples. In this work, we develop a quick, simple, and sensitive voltammetric method using chemically modified electrodes to determine PCT in complex matrices, including human serum and commercial solid formulations. We modify the carbon paste electrode with stevensite monoclinic clay mineral (Stv-CPE), using cyclic voltammetry, differential pulse voltammetry, and electrochemical impedance spectroscopy to characterise and detect PCT. The kinetics study provides a better electrochemical characterisation of the electrode behaviour, finding the detection and quantitation limits of 0.2 μM and 0.5 μM under favourable conditions. Further, the best linear working concentration range is 0.6-100 μM for PCT, applying the proposed method to the quantitative determination of PCT content in reference tablet formulations and biological samples for validation.
对乙酰氨基酚（PCT），又名乙酰氨基酚，是一种在全世界范围内广泛应用于多种临床用途的重要药物。然而，PCT 的过度或无差别使用可能会引发肝肾功能障碍；因此，确定生物样本中该目标物的含量至关重要。在这项工作中，我们开发了一种快速、简单、灵敏的伏安法，使用化学修饰电极来测定复杂基质中的 PCT，包括人血清和商业固体制剂。我们使用循环伏安法、差分脉冲伏安法和电化学阻抗谱法对单斜层状粘土矿物史蒂文斯石（Stv-CPE）进行修饰，以对 PCT 进行特征和检测。动力学研究提供了对电极行为更好的电化学表征，在有利条件下，检测限和定量限分别为 0.2 μM 和 0.5 μM。此外，在最佳线性工作浓度范围内，PCT 的浓度为 0.6-100 μM，该方法已应用于参考片剂制剂和生物样品中 PCT 含量的定量测定，以验证该方法的有效性。
