Gwangju Center, Korea Basic Science Institute, Gwangju 61751, Republic of Korea.
Department of Biomedical Sciences and Institute for Medical Science, Chonbuk National University Medical School, Jeonju 54907, Republic of Korea.
Int J Mol Sci. 2023 Jul 13;24(14):11392. doi: 10.3390/ijms241411392.
Given the impact of the gut microbiome on human physiology and aging, it is possible that the gut microbiome may affect locomotion in the same way as the host's own genes. There is not yet any direct evidence linking the gut microbiome to locomotion, though there are some potential connections, such as regular physical activity and the immune system. In this study, we demonstrate that the gut microbiome can contribute differently to locomotion. We remodeled the original gut microbiome of mice through fecal microbiota transplantation (FMT) using human feces and compared the changes in locomotion of the same mice before and three months after FMT. We found that FMT affected locomotion in three different ways: positive, none (the same), and negative. Analysis of the phylogenesis, α-diversities, and β-diversities of the gut microbiome in the three groups showed that a more diverse group of intestinal microbes was established after FMT in each of the three groups, indicating that the human gut microbiome is more diverse than that of mice. The FMT-remodeled gut microbiome in each group was also different from each other. Fold change and linear correlation analyses identified , , and in the gut microbiome as positive contributors to locomotion, while , , , and were found to have negative effects. This study not only confirms the presence of gut microbiomes that contribute differently to locomotion, but also explains the mixed results in research on the association between the gut microbiome and locomotion.
鉴于肠道微生物组对人类生理和衰老的影响，肠道微生物组可能以与宿主自身基因相同的方式影响运动。虽然有一些潜在的联系，如经常进行体育活动和免疫系统，但目前还没有直接证据将肠道微生物组与运动联系起来。在这项研究中，我们证明了肠道微生物组可以以不同的方式影响运动。我们通过使用人类粪便进行粪便微生物群移植（FMT）来重塑原始的小鼠肠道微生物群，并比较了 FMT 前后相同小鼠的运动变化。我们发现 FMT 以三种不同的方式影响运动：积极、无（相同）和消极。对三组肠道微生物群的系统发育、α多样性和β多样性进行分析表明，在每组中，FMT 后都建立了更多样化的肠道微生物群，这表明人类肠道微生物群比小鼠的肠道微生物群更加多样化。每组中经过 FMT 重塑的肠道微生物群彼此也不同。倍数变化和线性相关分析确定了 、 、 是对运动有积极贡献的肠道微生物群，而 、 、 、 和 被发现对运动有负面影响。这项研究不仅证实了存在对运动有不同贡献的肠道微生物群，还解释了肠道微生物群与运动之间关联的研究中存在的混合结果。
