Institute for Medicinal Plants Research "Dr Josif Pančić", Tadeuša Košćuška 1, 11000 Belgrade, Serbia.
Department of Plant Physiology, Institute for Biological Research "Siniša Stanković"-National Institute of Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11000 Belgrade, Serbia.
Int J Mol Sci. 2023 Jul 21;24(14):11764. doi: 10.3390/ijms241411764.
Mill. is a perennial herbaceous plant species, known for the medicinal value of all of its plant parts, although the chemical composition of the petals is unknown. This study aimed to determine the chemical fingerprint of the petals and also establish the optimal extraction parameters, extraction medium, and extraction method for petals collected from different localities in Serbia. The optimization was performed in order to acquire extracts that are rich in the contents of total polyphenol content (TPC) and total flavonoid content (TFC), and also exhibit strong antioxidant activity. In addition, the influence of the extracts on several human skin pathogens was evaluated, as well as their ability to aid wound closure and act as anti-inflammatory agents. Both the extraction medium and the applied technique significantly influenced the skin-beneficial biological activities, while methanol proved to be a more favorable extraction medium. In conclusion, the extraction conditions that yielded the extract with the richest phenolic content with satisfactory biological potential varied between the assays, while the most promising locality in Serbia for the collection of petals was Pančevo (South Banat).
曼陀罗是一种多年生草本植物，其所有植物部分都具有药用价值，尽管其花瓣的化学成分尚不清楚。本研究旨在确定花瓣的化学指纹图谱，并确定从塞尔维亚不同地区采集的花瓣的最佳提取参数、提取介质和提取方法。优化的目的是获得富含总多酚含量（TPC）和总黄酮含量（TFC）的提取物，并表现出较强的抗氧化活性。此外，还评估了提取物对几种人类皮肤病原体的影响，以及它们促进伤口闭合和作为抗炎剂的能力。提取介质和应用技术都对皮肤有益的生物活性有显著影响，而甲醇被证明是一种更有利的提取介质。总之，在不同的测定中，产生具有满意生物潜力的最丰富酚类含量提取物的提取条件有所不同，而塞尔维亚最有前途的采集花瓣的地区是潘切沃（南巴纳特）。
