Gudkov Sergey V, Gao Meng, Simakin Alexander V, Baryshev Alexey S, Pobedonostsev Roman V, Baimler Ilya V, Rebezov Maksim B, Sarimov Ruslan M, Astashev Maxim E, Dikovskaya Anastasia O, Molkova Elena A, Kozlov Valery A, Bunkin Nikolay F, Sevostyanov Mikhail A, Kolmakov Alexey G, Kaplan Mikhail A, Sharapov Mars G, Ivanov Vladimir E, Bruskov Vadim I, Kalinichenko Valery P, Aiyyzhy Kuder O, Voronov Valery V, Pimpha Nuttaporn, Li Ruibin, Shafeev Georgy A
Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilova St., 119991 Moscow, Russia.
Russian Scientific-Research Institute of Phytopathology of Russian Academy of Sciences, 143050 Big Vyazemy, Russia.
Materials (Basel). 2023 Jul 22;16(14):5164. doi: 10.3390/ma16145164.
With the help of laser ablation, a technology for obtaining nanosized crystalline selenium particles (SeNPs) has been created. The SeNPs do not exhibit significant toxic properties, in contrast to molecular selenium compounds. The administration of SeNPs can significantly increase the viabilities of SH-SY5Y and PCMF cells after radiation exposure. The introduction of such nanoparticles into the animal body protects proteins and DNA from radiation-induced damage. The number of chromosomal breaks and oxidized proteins decreases in irradiated mice treated with SeNPs. Using hematological tests, it was found that a decrease in radiation-induced leukopenia and thrombocytopenia is observed when selenium nanoparticles are injected into mice before exposure to ionizing radiation. The administration of SeNPs to animals 5 h before radiation exposure in sublethal and lethal doses significantly increases their survival rate. The modification dose factor for animal survival was 1.2. It has been shown that the introduction of selenium nanoparticles significantly normalizes gene expression in the cells of the red bone marrow of mice after exposure to ionizing radiation. Thus, it has been demonstrated that SeNPs are a new gene-protective and radioprotective agent that can significantly reduce the harmful effects of ionizing radiation.
借助激光烧蚀技术，已开发出一种制备纳米级结晶硒颗粒（SeNPs）的技术。与分子硒化合物不同，SeNPs不具有显著的毒性。给予SeNPs可显著提高辐射暴露后SH-SY5Y和PCMF细胞的活力。将此类纳米颗粒引入动物体内可保护蛋白质和DNA免受辐射诱导的损伤。在用SeNPs处理的受辐照小鼠中，染色体断裂和氧化蛋白质的数量减少。通过血液学检测发现，在小鼠暴露于电离辐射前注射硒纳米颗粒时，辐射诱导的白细胞减少和血小板减少有所减轻。在亚致死剂量和致死剂量辐射暴露前5小时给动物施用SeNPs可显著提高其存活率。动物存活的修正剂量因子为1.2。研究表明，在小鼠暴露于电离辐射后，引入硒纳米颗粒可显著使红骨髓细胞中的基因表达正常化。因此，已证明SeNPs是一种新型的基因保护和辐射防护剂，可显著降低电离辐射的有害影响。
