Medykowska Magdalena, Wiśniewska Małgorzata, Chibowski Stanisław
Department of Radiochemistry and Environmental Chemistry, Institute of Chemical Sciences, Faculty of Chemistry, Maria Curie-Sklodowska University in Lublin, M. Curie-Sklodowska Sq. 3, 20-031 Lublin, Poland.
Materials (Basel). 2023 Jul 24;16(14):5201. doi: 10.3390/ma16145201.
The main purpose of the investigations was to explore the protein adsorption on porous materials, as well as to identify the mechanisms of protein attachment without and with other common environmental contaminants, such as drugs, polymers or surfactants. This study applied the Na-X zeolite for the adsorption of bovine serum albumin (BSA) from solutions with various pH values. Electrophoretic mobility measurements and potentiometric titrations were conducted in systems containing both protein and/or PAA (poly(acrylic acid) polymer/DCF (diclofenac) drug/SDS (sodium dodecyl sulfate) surfactant to investigate the protein binding mechanisms in the complex adsorbate systems. In addition, aggregate size and stability measurements were performed in the investigated systems. Based on the research results, it was possible to conclude that the protein adsorbed most preferably on the zeolite surface at a pH value close to its isoelectric point (pI) (102.15 mg/g), and protein adsorption was the lowest in the solutions with strongly alkaline (29.61 mg/g) or acidic (77.45 mg/g) pH values. Thus, the examined zeolitic material can be considered an effective adsorbent for protein removal from an aqueous solution.
这些研究的主要目的是探索蛋白质在多孔材料上的吸附情况，以及确定在不存在和存在其他常见环境污染物（如药物、聚合物或表面活性剂）的情况下蛋白质附着的机制。本研究将Na-X沸石用于从不同pH值的溶液中吸附牛血清白蛋白（BSA）。在含有蛋白质和/或PAA（聚丙烯酸）聚合物/DCF（双氯芬酸）药物/SDS（十二烷基硫酸钠）表面活性剂的体系中进行电泳迁移率测量和电位滴定，以研究复合吸附质体系中的蛋白质结合机制。此外，还对所研究的体系进行了聚集体尺寸和稳定性测量。根据研究结果，可以得出结论：在接近其等电点（pI）的pH值下，蛋白质最优先吸附在沸石表面（102.15 mg/g），而在强碱性（29.61 mg/g）或酸性（77.45 mg/g）pH值的溶液中蛋白质吸附量最低。因此，所研究的沸石材料可被视为从水溶液中去除蛋白质的有效吸附剂。
