Max Planck Institute for Polymer Research, Mainz, Germany.
Macromol Biosci. 2011 May 12;11(5):628-38. doi: 10.1002/mabi.201000395. Epub 2011 Mar 7.
BSA adsorption onto negatively and positively charged polystyrene nanoparticles was investigated. The nanoparticles were characterized in terms of particle size, zeta potential, surface group density, and morphology. The adsorption behavior of BSA on the particle surface, as a function of pH and overall charge of the particle, was studied using ITC. Different thermodynamic data such as enthalpy changes upon binding and stoichiometry of the systems were determined and discussed. The degree of surface coverage with BSA was calculated using the thermodynamic data. The cellular uptake of particles before and after BSA adsorption was studied using HeLa cells in the presence and absence of supplemented FCS in the cell culture medium.
研究了带负电荷和带正电荷的聚苯乙烯纳米粒子对 BSA 的吸附。从粒径、zeta 电位、表面基团密度和形态等方面对纳米粒子进行了表征。使用 ITC 研究了 BSA 在粒子表面的吸附行为与 pH 值和粒子总电荷的关系。测定并讨论了结合过程中焓变和体系化学计量等不同热力学数据。使用热力学数据计算了 BSA 的表面覆盖率。通过 HeLa 细胞研究了在细胞培养基中添加和不添加补充 FCS 的情况下，BSA 吸附前后粒子的细胞摄取情况。
