Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains, Hubei Zhongke Research Institute of Industrial Technology, and College of Life Science, Huanggang Normal University, Huanggang 438000, Hubei Province, China.
J Biosci. 2021;46.
Flower color is the major characteristics and critical breeding program for most species. However, little is known about their coloration mechanism and color inheritance. In this study, petal pigment constituents of three Sweet cultivars with different colors were clarified based on LCESI- MS/MS method. Using a broad-targeted metabolomic approach, a total of 149 flavonoids and their glycosylated or methylated derivatives were identified, including 18 anthocyanins (Pg, Cy, Dp, Pn, Pt, and Mv) and 32 flavonols (mainly kaempferol 3-O-glycosides and quercetin 3-O-glycosides). Moreover, anthocyanins were mainly represented by anthocyanidin-3-O-glycosides (glucoside, rutinoside, galactoside, and di-glycosides). Flavone and C-glycosylated flavone were major second metabolites responsible for the difference among three different cultivars. The accumulation of total flavonoids displayed a clear phenotypic variation: cultivars 'zihe' and 'fenhe' were clustered together, while 'baihe' was clustered alone in the HCA analysis. The composition and content of anthocyanins were more complex in colored flowers ('zehe' and 'fenhe') than in white flower ('baihe'). This study further enhanced our understanding on the flavonoids profile of flower coloration and will provide biochemical basis for further genetic breeding in species.
花色是大多数物种的主要特征和关键育种计划。然而，它们的着色机制和颜色遗传知之甚少。在这项研究中，基于 LC-ESI-MS/MS 方法，阐明了三个具有不同颜色的甜品种的花瓣色素成分。使用广泛靶向的代谢组学方法，共鉴定出 149 种类黄酮及其糖基化或甲基化衍生物，包括 18 种花色苷（Pg、Cy、Dp、Pn、Pt 和 Mv）和 32 种类黄酮（主要为山奈酚 3-O-糖苷和槲皮素 3-O-糖苷）。此外，花色苷主要由花色苷-3-O-糖苷（葡萄糖苷、芦丁糖苷、半乳糖苷和二糖苷）组成。黄酮和 C-糖基化黄酮是导致三个不同品种差异的主要次生代谢物。总类黄酮的积累表现出明显的表型变异：品种“紫核”和“粉核”聚类在一起，而“白核”单独聚类在 HCA 分析中。有色花（“紫核”和“粉核”）中花色苷的组成和含量比白花（“白核”）更为复杂。本研究进一步加深了我们对花色黄酮谱的理解，并为进一步的遗传育种提供了生化基础。
