Cigane Urte, Palevicius Arvydas, Janusas Giedrius
Faculty of Mechanical Engineering and Design, Kaunas University of Technology, Studentu Street 56, 51424 Kaunas, Lithuania.
Micromachines (Basel). 2023 Jul 14;14(7):1419. doi: 10.3390/mi14071419.
Much attention has been paid to the surface modification of artificial skin barriers for the treatment of skin tissue damage. Chitosan is one of the natural materials that could be characterized by its biocompatibility. A number of methods for the preparation of chitosan membranes have been described in scientific articles, including solvent casting methods. This study investigates an improved technology to produce chitosan membranes. Thus, chitosan membranes were prepared using a vibration-assisted solvent casting method. First, aqueous acetic acid was used to pretreat chitosan. Then, free-standing chitosan membranes were prepared by solvent casting on nanoporous anodized aluminum oxide (AAO) membrane templates, allowing for the solvent to evaporate. Using finite element methods, a study was obtained showing the influence of chitosan solutions of different concentrations on the fluid flow into nanopores using high-frequency excitation. The height of the nanopillars and the surface area of the chitosan membrane were also evaluated. In this study, the surface area of the chitosan membrane was found to increase by 15, 10 and 6 times compared to the original flat surface area. The newly produced nanopillared chitosan membranes will be applicable in the fabrication of skin barriers due to the longer nanopillars on their surface and the larger surface area.
人工皮肤屏障的表面改性在皮肤组织损伤治疗方面受到了广泛关注。壳聚糖是一种具有生物相容性的天然材料。科学文献中描述了多种制备壳聚糖膜的方法，包括溶剂浇铸法。本研究探索了一种改进的制备壳聚糖膜的技术。因此，采用振动辅助溶剂浇铸法制备了壳聚糖膜。首先，用乙酸水溶液对壳聚糖进行预处理。然后，通过在纳米多孔阳极氧化铝（AAO）膜模板上进行溶剂浇铸制备独立的壳聚糖膜，使溶剂蒸发。利用有限元方法进行的一项研究表明，高频激发下不同浓度的壳聚糖溶液对流体流入纳米孔的影响。还评估了纳米柱的高度和壳聚糖膜的表面积。在本研究中，发现壳聚糖膜的表面积相较于原始平面表面积增加了15倍、10倍和6倍。新制备的具有纳米柱的壳聚糖膜因其表面更长的纳米柱和更大的表面积，将适用于皮肤屏障的制造。
