Dela Cruz Thomas Edison E, Behr Jan Helge, Geistlinger Joerg, Grosch Rita, Witzel Katja
Department of Biological Sciences, College of Science, University of Santo Tomas (UST), España Blvd., Manila 1015, Philippines.
Department of Plant-Microbes Systems, Leibniz Institute of Vegetables and Ornamental Crops (IGZ), Theodor-Echtermeyer-Weg 1, 14979 Groβbeeren, Germany.
Microorganisms. 2023 Jun 25;11(7):1655. doi: 10.3390/microorganisms11071655.
The persistence of beneficial microorganisms in the rhizosphere or surrounding soil following their application is a prerequisite for the effective interaction with the plant or indigenous microbial communities in the respective habitats. The goal of the study was to analyze the establishment and persistence of the applied beneficial (OMG16) strain in the maize root-associated soil depending on agricultural practice (soil management practice, N-fertilizer intensity) in a field experiment. A rapid identification of the inoculated strain OMG16 is essential for its monitoring. We used a culture-based approach coupled to matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis for the rapid identification of the inoculated strain as part of the beneficial microbe consortium (BMc). We isolated 428 fungal isolates from eight treatments of the field experiment. Forty eight percent of the isolated fungi equivalent to 205 fungal isolates were identified as , of which 87% (=179 isolates) were obtained from the fields inoculated with BMc. Gene sequence analysis showed a high similarity of the MALDI-TOF MS-identified , with that of the inoculated OMG16 confirming the re-isolation of the added beneficial fungus. This study highlighted the use of MALDI-TOF MS analysis as a quick, cost-effective detection and efficient monitoring tool for microbial-based bioinoculants in the field.
有益微生物施用于根际或周围土壤后在其中的持续存在，是其与相应生境中的植物或本地微生物群落进行有效相互作用的前提条件。该研究的目的是在田间试验中，根据农业实践（土壤管理实践、氮肥施用量）分析施用的有益（OMG16）菌株在玉米根际土壤中的定殖和持续存在情况。对接种的OMG16菌株进行快速鉴定对其监测至关重要。我们采用基于培养的方法并结合基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）分析，作为有益微生物联合体（BMc）一部分，对接种菌株进行快速鉴定。我们从田间试验的八个处理中分离出428株真菌分离物。所分离真菌的48%（相当于205株真菌分离物）被鉴定为，其中87%（=179株分离物）来自接种BMc的田地。基因序列分析表明，MALDI-TOF MS鉴定的与接种的OMG16高度相似，证实重新分离出了添加的有益真菌。本研究强调了MALDI-TOF MS分析可作为一种快速、经济高效的检测方法以及田间基于微生物的生物肥料的有效监测工具。
