Pulavarty Anusha, Horgan Karina, Kakouli-Duarte Thomais
Molecular Ecology and Nematode Research Group, enviroCORE, Department of Science and Health, Institute of Technology Carlow, Kilkenny Road, Carlow, Ireland.
Alltech Bioscience Centre, Dunboyne, County Meath, Ireland.
J Nematol. 2020;52:1-10. doi: 10.21307/jofnem-2020-014.
An organic product that consists of proprietary blend of fermentation and plant extracts with micronutrients (ACS 5075, Alltech, Inc., Nicholasville, KY USA) was evaluated against four strains of entomopathogenic nematodes (EPN): (SB12(1), a wild enviroCORE strain and a commercial form e-NEMA), (e-NEMA), and The effects on egg hatching and survival of root-knot nematodes (RKN) were also examined. The sensitivity to the product was tested by estimating mortality and survival of EPN infective juveniles (IJ) after 24-hr treatment with four different concentrations of product (4, 7, 8, and 10%) compared with the control in a 96-well plate. There was no significant difference in survival of IJ with 4% product compared to the control. A twofold reduction in survival was observed when the EPN were exposed to the product at 7%. A 10.5% RKN egg hatching was observed when RKN were exposed to 3% ACS 5075 concentration compared to 100% hatching in the untreated. A significant ( < 0.05) reduction in juvenile survival was observed at 0.5% treatment, however, it dropped to 0 when 1% and above was used for . Juveniles did not survive with all treatment concentrations in the case of The improvement in tomato plant growth and development were also observed when plants were treated with 1 and 3% ACS 5075. The product shows potential as a sustainable soil health alternative causing no harm to beneficial nematodes (EPN) at concentrations below 4%, while is promising against plant parasitic nematodes (PPN) and toward plant growth promotion. An organic product that consists of proprietary blend of fermentation and plant extracts with micronutrients (ACS 5075, Alltech, Inc., Nicholasville, KY USA) was evaluated against four strains of entomopathogenic nematodes (EPN): (SB12(1), a wild enviroCORE strain and a commercial form e-NEMA), (e-NEMA), and The effects on egg hatching and survival of root-knot nematodes (RKN) were also examined. The sensitivity to the product was tested by estimating mortality and survival of EPN infective juveniles (IJ) after 24-hr treatment with four different concentrations of product (4, 7, 8, and 10%) compared with the control in a 96-well plate. There was no significant difference in survival of IJ with 4% product compared to the control. A twofold reduction in survival was observed when the EPN were exposed to the product at 7%. A 10.5% RKN egg hatching was observed when RKN were exposed to 3% ACS 5075 concentration compared to 100% hatching in the untreated. A significant ( < 0.05) reduction in juvenile survival was observed at 0.5% treatment, however, it dropped to 0 when 1% and above was used for . Juveniles did not survive with all treatment concentrations in the case of The improvement in tomato plant growth and development were also observed when plants were treated with 1 and 3% ACS 5075. The product shows potential as a sustainable soil health alternative causing no harm to beneficial nematodes (EPN) at concentrations below 4%, while is promising against plant parasitic nematodes (PPN) and toward plant growth promotion.
对一种有机产品（由发酵物、植物提取物与微量营养素组成的专利配方产品，即美国肯塔基州尼古拉斯维尔市奥特奇公司的ACS 5075）进行了评估，以检测其对四种昆虫病原线虫（EPN）菌株的影响：（SB12(1)，一种野生环境核心菌株和一种商业形式的e-NEMA）、（e-NEMA）。同时还研究了该产品对根结线虫（RKN）卵孵化和存活的影响。通过在96孔板中用四种不同浓度（4%、7%、8%和10%）的产品处理24小时后，估计昆虫病原线虫感染性幼虫（IJ）的死亡率和存活率，来测试该产品的敏感性，并与对照组进行比较。与对照组相比，用4%产品处理时，IJ的存活率没有显著差异。当昆虫病原线虫暴露于7%的产品中时，存活率降低了两倍。当根结线虫暴露于3%的ACS 5075浓度下时，观察到10.5%的卵孵化率，而未处理组的孵化率为100%。在0.5%处理浓度下，观察到幼虫存活率显著（<0.05）降低，然而，当使用1%及以上浓度处理时，幼虫存活率降至0。在 情况下，所有处理浓度下幼虫均未存活。当用1%和3%的ACS 5075处理番茄植株时，也观察到植株生长和发育有所改善。该产品显示出作为一种可持续土壤健康替代品的潜力，在浓度低于4%时对有益线虫（EPN）无害，同时对植物寄生线虫（PPN）有防治前景，并能促进植物生长。对一种有机产品（由发酵物、植物提取物与微量营养素组成的专利配方产品，即美国肯塔基州尼古拉斯维尔市奥特奇公司的ACS 5075）进行了评估，以检测其对四种昆虫病原线虫（EPN）菌株的影响：（SB12(1)，一种野生环境核心菌株和一种商业形式的e-NEMA）、（e-NEMA）。同时还研究了该产品对根结线虫（RKN）卵孵化和存活的影响。通过在96孔板中用四种不同浓度（4%、7%、8%和10%）的产品处理24小时后，估计昆虫病原线虫感染性幼虫（IJ）的死亡率和存活率，来测试该产品的敏感性，并与对照组进行比较。与对照组相比，用4%产品处理时，IJ的存活率没有显著差异。当昆虫病原线虫暴露于7%的产品中时，存活率降低了两倍。当根结线虫暴露于3%的ACS 5075浓度下时，观察到10.5%的卵孵化率，而未处理组的孵化率为100%。在0.5%处理浓度下，观察到幼虫存活率显著（<0.05）降低，然而，当使用1%及以上浓度处理时，幼虫存活率降至0。在 情况下，所有处理浓度下幼虫均未存活。当用1%和3%的ACS 5075处理番茄植株时，也观察到植株生长和发育有所改善。该产品显示出作为一种可持续土壤健康替代品的潜力，在浓度低于4%时对有益线虫（EPN）无害，同时对植物寄生线虫（PPN）有防治前景，并能促进植物生长。
