乳酸菌对黄曲霉毒素B1致大鼠器官毒性的保护和治疗作用

Protective and Therapeutic Effects of Lactic Acid Bacteria against Aflatoxin B1 Toxicity to Rat Organs.

作者信息

Ashi Hayat, Almalki Meshal H K, Hamed Enas A, Ramadan Wafaa S, Alahmadi Tahani F H, Alami Outour Tariq, Arafa Sara H, Alshareef Atheer K, Alsulami Fatimah S, Alharbi Areej F, Al-Harbi Manahil S, Alqurashi Ebtehal H, Aashi Shirin, Alzahrani Youssef A, Elbanna Khaled, Abulreesh Hussein H

机构信息

Department of Biology, Faculty of Applied Science, Umm Al-Qura University, Makkah 21955, Saudi Arabia.

Research Laboratories Unit, Faculty of Applied Science, Umm Al-Qura University, Makkah 21955, Saudi Arabia.

出版信息

Microorganisms. 2023 Jun 29;11(7):1703. doi: 10.3390/microorganisms11071703.

DOI:10.3390/microorganisms11071703

PMID:37512876

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10385160/

Abstract

BACKGROUND

Aflatoxin (AF), a metabolite of , is injurious to vital body organs. The bacterial defense against such mycotoxins has attracted significant attention. Lactic acid bacteria (LAB) are known to ameliorate AF toxicity.

METHODS

Thirty adult male rats were divided into six groups (five each) to perform the experiments. The control (Co) group was fed a basal diet and water. Each of the following periods lasted 21 days: the milk (MK) group orally received milk (500 µL); LAB suspension (500 µL) containing 10 cfu/mL was orally provided to the LAB group; AF (0.5 mg/kg) was orally given to the AF group; and a combination of AF and LAB was administered to the AF + LAB group. The AF/LAB group was initially given AF for 21 days, followed by LAB for the same period. Finally, the rats were dissected to retrieve blood and tissue samples for hematological, biochemical, and histological studies.

RESULTS

The results revealed a significant decrease in RBCs, lymphocytes, total proteins, eosinophil count, albumin, and uric acid, whereas the levels of WBCs, monocytes, neutrophils, creatinine, urea, aspartate aminotransferase, alkaline phosphatase, alanine aminotransferase, lactate dehydrogenase, and creatinine kinase significantly increased in the AF group in comparison to the control group. The histological examination of the AF group revealed necrosis and apoptosis of the kidney's glomeruli and renal tubules, nuclei vacuolization and apoptosis of hepatocytes, congestion of the liver's dilated portal vein, lymphoid depletion in the white pulp, localized hemorrhages, hemosiderin pigment deposition in the spleen, and vacuolization of seminiferous tubules with a complete loss of testis spermatogenic cells. Meanwhile, protective and therapeutic LAB administration in AF-treated rats improved the hematological, biochemical, and histological changes.

CONCLUSIONS

The study revealed LAB-based amelioration to AFB1-induced disruptions of the kidney, liver, spleen, and testis by inhibiting tissue damage. The therapeutic effects of LAB were comparatively more pronounced than the protective effects.

摘要

背景

黄曲霉毒素（AF）是的一种代谢产物，对人体重要器官有害。细菌对这类霉菌毒素的防御作用已引起广泛关注。已知乳酸菌（LAB）可减轻AF的毒性。

方法

将30只成年雄性大鼠分为六组（每组五只）进行实验。对照组（Co）给予基础饮食和水。以下每个阶段持续21天：牛奶（MK）组口服牛奶（500微升）；LAB组口服含10 cfu/mL的LAB悬液（500微升）；AF组口服AF（0.5毫克/千克）；AF + LAB组给予AF和LAB的组合。AF/LAB组最初给予AF 21天，随后给予LAB相同时间。最后，解剖大鼠以获取血液和组织样本用于血液学、生化和组织学研究。

结果

结果显示，与对照组相比，AF组的红细胞、淋巴细胞、总蛋白、嗜酸性粒细胞计数、白蛋白和尿酸显著降低，而白细胞、单核细胞、中性粒细胞、肌酐、尿素、天冬氨酸转氨酶、碱性磷酸酶、丙氨酸转氨酶、乳酸脱氢酶和肌酸激酶水平显著升高。AF组的组织学检查显示肾小球和肾小管坏死及凋亡、肝细胞细胞核空泡化和凋亡、肝门静脉扩张充血、白髓淋巴细胞耗竭、局部出血、脾脏含铁血黄素色素沉积以及生精小管空泡化且睾丸生精细胞完全丧失。同时，在AF处理的大鼠中给予具有保护和治疗作用的LAB可改善血液学、生化和组织学变化。