Ye Jinling, Zhang Chang, Fan Qiuli, Lin Xiajing, Wang Yibing, Azzam Mahmoud, Alhotan Rashed, Alqhtani Abdulmohsen, Jiang Shouqun
State Key Laboratory of Livestock and Poultry Breeding, Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Provincial Key Laboratory of Animal Breeding and Nutrition, Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
Department of Animal Production College of Food and Agriculture Sciences, King Saud University, Riyadh, Saudi Arabia.
Front Vet Sci. 2022 Oct 10;9:994782. doi: 10.3389/fvets.2022.994782. eCollection 2022.
Lipopolysaccharides (LPS) induces liver inflammatory response by activating the TLR4/NF-κB signaling pathway. polysaccharide (ACP) is a medicinal mushroom that can protect from intoxication, liver injury, and inflammation. Nevertheless, the effect of ACP on the liver antioxidant, anti-inflammatory capacity and cecal flora structure of LPS-challenged broilers remains unclear. The aim of this experiment was to investigate the effects of ACP on the anti-oxidative and anti-inflammatory capacities of the liver, and cecal microbiota in slow-growing broilers stimulated by LPS. A total of 750 slow-growing broilers (9-day-old) were assigned to five treatments with 6 replicates of 25 chicks per replicate: a control diet, the chicks were fed a control diet and challenged with LPS. Dietary treatments 3 to 5 were the control diet supplemented with 100, 200, 400 mg/kg ACP challenged with LPS, respectively. The groups of 100 mg/kg ACP supplementation significantly increased liver index, pancreas index, and bursa of Fabricius index ( < 0.05). The GSH-Px content of LPS-challenged broilers was lower than that of the control group ( < 0.001), but the content of MDA increased ( < 0.001). Feeding with 100 mg/kg ACP resulted in increased the activity of T-AOC, GSH-Px, and T-SOD, and decreased MDA content ( < 0.05). The activity of TNF-α, IL-1β, and IL-6 of the LPS group increased, but these indicators were decreased with supplemental 100 mg/kg ACP ( < 0.05). Dietary application of ACP up to 100 mg/kg down-regulated ( < 0.05) the expression of TLR4/NF-κB pathway in the liver induced by LPS. The results of 16S rRNA demonstrated that feeding with 100 mg/kg ACP can change the diversity and composition of the gut microbiota, and restrained the decline of beneficial cecal microbiota (typically , and group) in the challenged LPS group ( < 0.05). Conclusively, feeding a diet with 100 mg/kg ACP may have beneficial effects on liver damage and the bacterial microbiota diversity and composition in the ceca of LPS-stressed slow-growing broiler breeds, probably because of its combined favorable effects on antioxidants and cytokines contents, and restoration the decline of beneficial cecal microbiota.
脂多糖（LPS）通过激活TLR4/NF-κB信号通路诱导肝脏炎症反应。姬松茸多糖（ACP）是一种药用真菌，具有解毒、保护肝脏免受损伤和抗炎作用。然而，ACP对LPS刺激的肉鸡肝脏抗氧化、抗炎能力及盲肠菌群结构的影响尚不清楚。本试验旨在研究ACP对LPS刺激的慢生长肉鸡肝脏抗氧化和抗炎能力以及盲肠微生物群的影响。选取750只9日龄慢生长肉鸡，分为5个处理组，每组6个重复，每个重复25只鸡：对照组饲喂基础日粮，然后用LPS攻毒。日粮处理3至5为在基础日粮中分别添加100、200、400 mg/kg ACP后用LPS攻毒。添加100 mg/kg ACP组显著提高了肝脏指数、胰腺指数和法氏囊指数（P<0.05）。LPS攻毒肉鸡的谷胱甘肽过氧化物酶（GSH-Px）含量低于对照组（P<0.001），但丙二醛（MDA）含量升高（P<0.001）。饲喂100 mg/kg ACP可提高总抗氧化能力（T-AOC）、GSH-Px和总超氧化物歧化酶（T-SOD）活性，降低MDA含量（P<0.05）。LPS组肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）和白细胞介素-6（IL-6）活性升高，但添加100 mg/kg ACP后这些指标降低（P<0.05）。日粮中添加高达100 mg/kg的ACP可下调LPS诱导的肝脏中TLR4/NF-κB信号通路的表达（P<0.05）。16S rRNA结果表明，饲喂100 mg/kg ACP可改变肠道微生物群的多样性和组成，并抑制LPS攻毒组有益盲肠微生物群（典型的如，和组）数量的下降（P<0.05）。综上所述，在LPS应激的慢生长肉鸡日粮中添加100 mg/kg ACP可能对肝脏损伤以及盲肠细菌微生物群的多样性和组成具有有益影响，这可能是由于其对抗氧化剂和细胞因子含量具有综合有利影响，并恢复了有益盲肠微生物群数量的下降。
