Tan Xiqian, Wang Xiaoqing, Cui Fangchao, Zeshan Ali, Wang Dangfeng, Li Xuepeng, Li Jianrong
College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China.
College of Food Science and Engineering, Bohai University, Jinzhou 121013, China.
Molecules. 2024 Dec 8;29(23):5800. doi: 10.3390/molecules29235800.
The sea cucumber intestine (SI), a secondary product from sea cucumber processing, contains polysaccharides as one of its active ingredients, and fermentation is an effective method for extracting bioactive substances from food by-products. In this study, to explore the effect of GS22 fermentation on the extraction of SI polysaccharides, the polysaccharides were extracted through the SI with and without GS22 fermentation, and the obtained polysaccharides were designated as SC-PF and SC-P. The extraction yield, the structural characteristics, and the biological functions of the polysaccharides were then evaluated. The results indicated that GS22 could grow well using SI as the substrate and that fermentation could improve the extraction yield of the polysaccharide from 0.48% to 0.63%, decrease the molecular weight (Mw), and change the monosaccharide composition. The diameter of SC-PF was smaller than SC-P, and the absolute value of the zeta potential of SC-PF was found to be lower than SC-P. Fermentation does not change the functional group or the thermal ability of the polysaccharide. SC-PF had better antioxidant ability than SC-P; the DPPH and superoxide anion scavenging ability were 96.3% and 36.5%, respectively. SC-PF also showed nearly 1.3- and 1.1-fold higher inhibition of α-glucosidase and α-amylase as compared to SC-P. The current results showed that GS22 fermentation has the potential to extract SI polysaccharides with better prebiotic abilities.
海参肠是海参加工的副产品，其活性成分之一为多糖，发酵是从食品副产品中提取生物活性物质的有效方法。本研究为探究GS22发酵对海参肠多糖提取的影响，分别对经GS22发酵和未经GS22发酵的海参肠进行多糖提取，所得多糖分别命名为SC-PF和SC-P。随后对多糖的提取率、结构特征及生物学功能进行评估。结果表明，GS22能够以海参肠为底物良好生长，发酵可使多糖提取率从0.48%提高至0.63%，降低分子量（Mw），并改变单糖组成。SC-PF的粒径小于SC-P，且SC-PF的ζ电位绝对值低于SC-P。发酵未改变多糖的官能团或热性能。SC-PF的抗氧化能力优于SC-P；其对DPPH和超氧阴离子的清除能力分别为96.3%和36.5%。与SC-P相比，SC-PF对α-葡萄糖苷酶和α-淀粉酶的抑制作用分别高出近1.3倍和1.1倍。目前的结果表明，GS22发酵具有提取具有更好益生元能力的海参肠多糖的潜力。
