Department of Food Science and Biotechnology, Kyungpook National University 80, Daehakro, Bukgu, Daegu 702701, Republic of Korea.
Nutrients. 2023 Jul 8;15(14):3078. doi: 10.3390/nu15143078.
During the worldwide COVID-19 outbreak, there was an increase in the prevalence of obesity, including childhood obesity, due to which the awareness of obesity and interest in treatment increased. Accordingly, we describe EJF ( Kanitz fruit) extract as a candidate for naturally derived antiobesity agents. In this study, we found that EJF is involved in the early stage of adipogenic differentiation in vitro and finally inhibits adipogenesis. We propose two mechanisms for the antiobesity effect of EJF. First, EJF inhibits MDI-induced mitotic clonal expansion (MCE) by inducing cell cycle arrest at the initiation of adipogenic differentiation. The second aims to regulate stability and activation at the protein level of IRS1, which initiates differentiation in the early stage of differentiation. As a result, it was found that the activation of Akt decreased, leading to the inhibition of the expression of adipogenesis-related transcription factors (PPARγ, C/EBPα) and the subsequent suppression of adipogenic differentiation. In summary, we suggest that EJF can inhibit adipogenesis and lipid accumulation by suppressing the early stage of adipogenic differentiation in 3T3-L1 adipocytes. These findings indicate that EJF's functionality could be beneficial in the treatment of obesity, particularly childhood obesity associated with adipocyte hyperplasia.
在全球 COVID-19 疫情期间，肥胖症（包括儿童肥胖症）的患病率有所上升，因此，人们对肥胖症的认识和对治疗的兴趣有所增加。相应地，我们将 EJF（Kanitz 果实）提取物描述为天然来源的抗肥胖药物候选物。在这项研究中，我们发现 EJF 参与体外脂肪生成分化的早期阶段，最终抑制脂肪生成。我们提出了 EJF 抗肥胖作用的两种机制。首先，EJF 通过在脂肪生成分化起始时诱导细胞周期停滞来抑制 MDI 诱导的有丝分裂克隆扩张（MCE）。第二个目的是调节 IRS1 的稳定性和激活，IRS1 在分化的早期阶段启动分化。结果发现，Akt 的激活减少，导致与脂肪生成相关的转录因子（PPARγ、C/EBPα）的表达受到抑制，随后抑制脂肪生成分化。综上所述，我们认为 EJF 可以通过抑制 3T3-L1 脂肪细胞中脂肪生成分化的早期阶段来抑制脂肪生成和脂质积累。这些发现表明，EJF 的功能可能有益于肥胖症的治疗，特别是与脂肪细胞增生相关的儿童肥胖症。
