Laboratory of Nutrition and Development, Key Laboratory of Major Diseases in Children, Ministry of Education, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing 100045, China.
Department of Children's Health Care Center, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing 100045, China.
Nutrients. 2023 Jul 19;15(14):3198. doi: 10.3390/nu15143198.
Antibiotic resistance genes (ARGs) threaten the success of modern drugs against multidrug resistant infections. ARGs can be transferred to opportunistic pathogens by horizontal gene transfer (HGT). Many studies have investigated the characteristics of ARGs in various chemical stressors. Studies on the effects of dietary nutrition and dietary patterns on ARGs are rare. The study first demonstrated the effect of calorie restricted (CR) diet on the ARGs and mobile genetic elements (MGEs) in mouse feces and explored their relationship with gut microbiota and their functions. The results showed that the abundance of the total ARGs in mouse feces of the CR group increased, especially tetracycline ARGs (W-01). The abundance of the MLSB ARGs (B) decreased evidently in mouse feces of the CR group. In addition, the total abundance of MGEs decreased evidently in the CR group, especially A-03. In the meantime, the abundance of and in mouse feces of the CR group increased remarkably. The Spearman correlation analysis between gut microbiota and ARGs showed that several probiotics were significantly positively correlated with ARGs (W-01), which might be the main contribution to the increase in ARGs of the CR group.
抗生素耐药基因（ARGs）威胁着现代药物对抗多重耐药感染的成功。ARGs 可以通过水平基因转移（HGT）转移到机会性病原体。许多研究已经研究了各种化学应激物中 ARGs 的特征。关于饮食营养和饮食模式对 ARGs 的影响的研究很少。该研究首次证明了限食（CR）饮食对小鼠粪便中 ARGs 和可移动遗传元件（MGE）的影响，并探讨了它们与肠道微生物群及其功能的关系。结果表明，CR 组小鼠粪便中总 ARGs 的丰度增加，特别是四环素类 ARGs（W-01）。CR 组小鼠粪便中 MLSB ARGs（B）的丰度明显下降。此外，CR 组中 MGEs 的总丰度明显下降，特别是 A-03。与此同时，CR 组小鼠粪便中 和 的丰度显著增加。肠道微生物群与 ARGs 的 Spearman 相关分析表明，几种益生菌与 ARGs（W-01）呈显著正相关，这可能是 CR 组 ARGs 增加的主要原因。
