Tuttle James T, Bruce Timothy J, Butts Ian A E, Roy Luke A, Abdelrahman Hisham A, Beck Benjamin H, Kelly Anita M
Alabama Fish Farming Center, Greensboro, AL 36744, USA.
School of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, Auburn, AL 36849, USA.
Pathogens. 2023 Jun 25;12(7):871. doi: 10.3390/pathogens12070871.
Two prevalent bacterial diseases in catfish aquaculture are enteric septicemia of catfish and columnaris disease caused by and , respectively. Chronic and recurring outbreaks of these bacterial pathogens result in significant economic losses for producers annually. Determining if these pathogens can persist within sediments of commercial ponds is paramount. Experimental persistence trials (PT) were conducted to evaluate the persistence of and in pond sediments. Twelve test chambers containing 120 g of sterilized sediment from four commercial catfish ponds were inoculated with either (S97-773) or (ALG-00-530) and filled with 8 L of disinfected water. At 1, 2, 4-, 6-, 8-, and 15-days post-inoculation, 1 g of sediment was removed, and colony-forming units (CFU) were enumerated on selective media using 6 × 6 drop plate methods. population peaked on Day 3 at 6.4 ± 0.5 log CFU g. Correlation analysis revealed no correlation between the sediment physicochemical parameters and log CFU g. However, no viable colonies were recovered after two PT attempts. Future studies to improve understanding of pathogenesis and persistence, and potential persistence in pond bottom sediments are needed.
鲶鱼养殖中两种常见的细菌性疾病分别是由[具体细菌名称1]引起的鲶鱼肠道败血症和由[具体细菌名称2]引起的柱状病。这些细菌病原体的慢性和反复爆发每年给养殖户造成重大经济损失。确定这些病原体是否能在商业池塘的沉积物中持续存在至关重要。进行了实验性持久性试验（PT）以评估[具体细菌名称1]和[具体细菌名称2]在池塘沉积物中的持久性。十二个测试室装有来自四个商业鲶鱼池塘的120克灭菌沉积物，分别接种[具体细菌名称1]（S97 - 773）或[具体细菌名称2]（ALG - 00 - 530），并加入8升消毒水。在接种后第1、2、4、6、8和15天，取出1克沉积物，使用6×6滴板法在选择性培养基上计数菌落形成单位（CFU）。[具体细菌名称1]种群在第3天达到峰值，为6.4±0.5 log CFU/g。相关性分析表明沉积物理化参数与[具体细菌名称1] log CFU/g之间无相关性。然而，经过两次PT尝试后未回收 viable [具体细菌名称2]菌落。需要进一步研究以更好地理解[具体细菌名称2]的发病机制和持久性，以及其在池塘底部沉积物中的潜在持久性。
