Cui Dapeng, Li Ming, Zhang Peng, Rao Feng, Huang Wei, Wang Chuanlin, Guo Wei, Wang Tianbing
Hepatobiliary Surgery Department, The First Affiliated Hospital of Hebei North University, Zhangjiakou 075000, China.
Trauma Medicine Center, Peking University People's Hospital, Beijing 100044, China.
Polymers (Basel). 2023 Jul 22;15(14):3122. doi: 10.3390/polym15143122.
Hemorrhagic shock is the primary cause of death in patients with severe trauma, and the development of rapid and efficient hemostatic methods is of great significance in saving the lives of trauma patients. In this study, a polycaprolactone (PCL) nanofiber membrane was prepared by electrospinning. A PCL-PDA loading system was developed by modifying the surface of polydopamine (PDA), using inspiration from mussel adhesion protein, and the efficient and stable loading of thrombin (TB) was realized to ensure the bioactivity of TB. The new thrombin loading system overcomes the disadvantages of harsh storage conditions, poor strength, and ease of falling off, and it can use thrombin to start a rapid coagulation cascade reaction, which has the characteristics of fast hemostasis, good biocompatibility, high safety, and a wide range of hemostasis. The physicochemical properties and biocompatibility of the PCL-PDA-TB membrane were verified by scanning electron microscopy, the cell proliferation test, the cell adhesion test, and the extract cytotoxicity test. Red blood cell adhesion, platelet adhesion, dynamic coagulation time, and animal models all verified the coagulation effect of the PCL-PDA-TB membrane. Therefore, the PCL-PDA-TB membrane has great potential in wound hemostasis applications, and should be widely used in various traumatic hemostatic scenarios.
失血性休克是严重创伤患者死亡的主要原因，开发快速有效的止血方法对于挽救创伤患者的生命具有重要意义。在本研究中，通过静电纺丝制备了聚己内酯（PCL）纳米纤维膜。受贻贝粘附蛋白的启发，通过修饰聚多巴胺（PDA）的表面开发了一种PCL-PDA负载系统，实现了凝血酶（TB）的高效稳定负载，以确保TB的生物活性。新型凝血酶负载系统克服了储存条件苛刻、强度差和易脱落的缺点，并且可以利用凝血酶启动快速的凝血级联反应，具有止血迅速、生物相容性好、安全性高和止血范围广的特点。通过扫描电子显微镜、细胞增殖试验、细胞粘附试验和提取物细胞毒性试验对PCL-PDA-TB膜的理化性质和生物相容性进行了验证。红细胞粘附、血小板粘附、动态凝血时间和动物模型均验证了PCL-PDA-TB膜的凝血效果。因此，PCL-PDA-TB膜在伤口止血应用中具有巨大潜力，应广泛应用于各种创伤止血场景。
