School of Pharmacy, Nanjing Medical University, Nanjing, 211166, Jiangsu, China.
Mikrochim Acta. 2023 Jul 29;190(8):336. doi: 10.1007/s00604-023-05900-1.
A novel magnetic nanozyme FeO@MXene-Au nanocomposite, which possessed higher peroxidase-like activity than that of FeO nanoparticles and FeO@MXene nanocomposites, was developed. The outstanding magnetic properties of the nanozyme endowed it with the ability of simple and rapid separation, achieving great recyclability. Based on FeO@MXene-Au nanocomposites and glucose oxidase (Glu Ox), a highly selective colorimetric biosensor for glucose detection was developed. FeO@MXene-Au nanocomposites can catalyze HO produced from glucose catalyzed by glucose oxidase to ·OH and oxidize colorless 3,3',5,5'-tetramethylbenzidine (TMB) to blue oxidized TMB (oxTMB) with a significant absorbance at 652 nm. The linear range of glucose was 0-1.4 mM under optimal conditions, with a limit of detection (LOD) of 0.11 mM. Glucose in human whole blood was successfully detected with satisfactory recoveries. Furthermore, a facile agarose hydrogel detection platform was designed. With smartphone software, glucose detection can be realized by the agarose hydrogel platform, demonstrating the potential in on-site and visual detection of glucose.
开发了一种新型的磁性纳米酶 FeO@MXene-Au 纳米复合材料，其过氧化物酶样活性高于 FeO 纳米粒子和 FeO@MXene 纳米复合材料。该纳米酶具有优异的磁性，使其能够简单快速地分离，实现了很好的可循环性。基于 FeO@MXene-Au 纳米复合材料和葡萄糖氧化酶（GluOx），开发了一种用于葡萄糖检测的高选择性比色生物传感器。FeO@MXene-Au 纳米复合材料可以催化葡萄糖氧化酶催化生成的 HO 生成·OH，并将无色的 3,3',5,5'-四甲基联苯胺（TMB）氧化为蓝色的氧化 TMB（oxTMB），在 652nm 处有明显的吸光度。在最佳条件下，葡萄糖的线性范围为 0-1.4mM，检测限（LOD）为 0.11mM。成功地检测了人全血中的葡萄糖，回收率令人满意。此外，还设计了一种简单的琼脂糖水凝胶检测平台。通过智能手机软件，可以利用琼脂糖水凝胶平台实现葡萄糖检测，展示了在现场和可视化检测葡萄糖方面的潜力。
