通过热核酸酶试验对金黄色葡萄球菌菌血症进行当日确认。

Same-day confirmation of Staphylococcus aureus bacteraemia by a thermonuclease test.

作者信息

Bergh K, Maeland J A

出版信息

Acta Pathol Microbiol Immunol Scand B. 1986 Aug;94(4):291-2. doi: 10.1111/j.1699-0463.1986.tb03055.x.

DOI:10.1111/j.1699-0463.1986.tb03055.x

Abstract

A test to detect thermostable DNase activity (TDNase test) was evaluated for rapid confirmation of S. aureus bacteraemia, using toluidine blue O DNA agar plates for the testing. A total of 226 blood cultures which grew bacteria were examined. S. aureus was identified in 76 of the cultures. All of the S. aureus isolates showed positive TDNase test after 2 h of incubation of the test plates. The remaining isolates examined, most of them coagulase-negative staphylococci, showed a negative test. The TDNase test enables reliable same-day confirmation of S. aureus bacteraemia.

摘要

使用甲苯胺蓝O DNA琼脂平板进行检测，对一种用于快速确认金黄色葡萄球菌菌血症的检测耐热DNA酶活性的试验（TDNase试验）进行了评估。共检查了226份培养出细菌的血培养物。在76份培养物中鉴定出金黄色葡萄球菌。将试验平板培养2小时后，所有金黄色葡萄球菌分离株的TDNase试验均呈阳性。其余检测的分离株，大多数为凝固酶阴性葡萄球菌，试验呈阴性。TDNase试验能够可靠地在同一天确认金黄色葡萄球菌菌血症。