Ratner H B, Stratton C W
J Clin Microbiol. 1985 Jun;21(6):995-6. doi: 10.1128/jcm.21.6.995-996.1985.
We used a thermonuclease test to presumptively identify Staphylococcus aureus directly in blood cultures on the same day that a smear of the broth showed gram-positive cocci in clusters. There were no discrepancies between the identification of S. aureus directly from 250 blood cultures and identification by the tube coagulase test which was performed on the 18- to 24-h subculture isolates. These rapid results were clinically relevant and enabled physicians to make more timely and cost-effective decisions about antibiotic therapy.
在肉汤涂片显示革兰氏阳性球菌呈簇状的同一天，我们使用热核酸酶试验直接在血培养物中初步鉴定金黄色葡萄球菌。直接从250份血培养物中鉴定金黄色葡萄球菌与对18至24小时传代培养分离株进行的试管凝固酶试验鉴定结果之间没有差异。这些快速结果具有临床相关性，使医生能够就抗生素治疗做出更及时且具成本效益的决策。
