Department of Surgery, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, SP, Brazil.
Department of Preventive Veterinary Medicine and Animal Reproduction, Faculty of Agricultural and Veterinary Sciences, State University of São Paulo, Jaboticabal, SP, Brazil.
Tissue Cell. 2023 Oct;84:102181. doi: 10.1016/j.tice.2023.102181. Epub 2023 Jul 26.
Hepatic microenvironment plays an essential role in liver regeneration, providing the necessary conditions for cell proliferation, differentiation and tissue rearrangement. One of the key factors for hepatic tissue reconstruction is the extracellular matrix (ECM), which through collagenous and non-collagenous proteins provide a three-dimensional structure that confers support for cell adhesion and assists on their survival and maintenance. In this scenario, placental ECM may be eligible for hepatic tissue reconstruction, once these scaffolds hold the major components required for cell support. Therefore, this preliminary study aimed to access the possibility of mouse embryonic stem cells differentiation into hepatocyte-like cells on placental scaffolds in a three-dimensional dynamic system using a Rotary Cell Culture System. Following a four-phase differentiation protocol that simulates liver embryonic development events, the preliminary results showed that a significant quantity of cells adhered and interacted with the scaffold through outer and inner surfaces. Positive immunolabelling for alpha fetus protein and CK7 suggest presence of hepatoblast phenotype cells, and CK18 and Albumin positive immunolabelling suggest the presence of hepatocyte-like phenotype cells, demonstrating the presence of a heterogeneous population into the recellularized scaffolds. Periodic Acid Schiff-Diastase staining confirmed the presence of glycogen storage, indicating that differentiate cells acquired a hepatic-like phenotype. In conclusion, these preliminary results suggested that mouse placental scaffolds might be used as a biological platform for stem cells differentiation into hepatic-like cells and their establishment, which may be a promissing biomaterial for hepatic tissue reconstruction.
肝微环境在肝脏再生中起着至关重要的作用，为细胞增殖、分化和组织重排提供必要的条件。肝组织重建的一个关键因素是细胞外基质 (ECM)，它通过胶原和非胶原蛋白提供三维结构，为细胞黏附提供支持，并有助于细胞的存活和维持。在这种情况下，胎盘 ECM 可能有资格用于肝组织重建，因为这些支架包含支持细胞所需的主要成分。因此，本初步研究旨在使用旋转细胞培养系统，在三维动态系统中评估小鼠胚胎干细胞在胎盘支架上向肝样细胞分化的可能性。通过模拟肝胚胎发育事件的四阶段分化方案，初步结果表明，大量细胞通过外表面和内表面与支架黏附和相互作用。α胎蛋白和 CK7 的免疫阳性标记表明存在肝母细胞表型细胞，CK18 和白蛋白的免疫阳性标记表明存在肝样细胞表型细胞，证明再细胞化支架中存在异质群体。过碘酸希夫-二淀粉酶染色证实存在糖原储存，表明分化细胞获得了肝样表型。总之，这些初步结果表明，小鼠胎盘支架可用作干细胞向肝样细胞分化及其建立的生物平台，这可能是肝组织重建的一种有前途的生物材料。
