Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun, 130012, PR China.
Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun, 130012, PR China.
Talanta. 2024 Jan 1;266(Pt 1):124991. doi: 10.1016/j.talanta.2023.124991. Epub 2023 Jul 25.
Herein, a novel peroxidase-like (POD-like) nanozyme Cu/B-NC was synthesized. The Cu and B co-doped nanozyme Cu/B-NC has competitive POD-like activity but negligible oxidase-like (OXD-like) activity, which is proved to partly benefit from the doping of boron atom. The catalytic activity of Cu/B-NC is high with great affinity for TMB and HO and high reaction velocity. Cu/B-NC was utilized to catalyze the condensation of phenolic substance epinephrine (EP) and 4-aminoantipyrine (4-AAP) to form colored quinone imine in the presence of HO. UV-vis absorbance of quinone imine at 492 nm was used for EP determination. Catalase (CAT) could decompose HO, so CAT could also be quantified through absorbance variation. The linear ranges of colorimetric detection for EP and CAT were 2-100 μM and 1-30 U mL, respectively. The limits of detection (LODs) for EP and CAT were 0.97 μM and 0.57 U mL, respectively. The practicability of this sensing platform was further validated by successful application in actual samples.
本文合成了一种新型过氧化物酶样（POD-like）纳米酶 Cu/B-NC。Cu 和 B 共掺杂的纳米酶 Cu/B-NC 具有竞争性的过氧化物酶样（POD-like）活性，但几乎没有氧化酶样（OXD-like）活性，这部分归因于硼原子的掺杂。Cu/B-NC 的催化活性高，对 TMB 和 HO 具有很大的亲和力和高反应速度。Cu/B-NC 可在 HO 的存在下催化酚类物质肾上腺素（EP）和 4-氨基安替比林（4-AAP）缩合形成有色醌亚胺，醌亚胺在 492nm 处有紫外可见吸收。通过测定醌亚胺的吸光度来检测 EP。过氧化氢酶（CAT）可以分解 HO，因此通过吸光度变化也可以定量 CAT。比色法检测 EP 和 CAT 的线性范围分别为 2-100μM 和 1-30U mL，检测限（LOD）分别为 0.97μM 和 0.57U mL。该传感平台的实用性通过在实际样品中的成功应用得到了进一步验证。
