Department of General Surgery, Hui Ya Hospital of The First Affiliated Hospital, Sun Yat-Sen University, Huizhou, 516081, Guangdong, China.
Central Laboratory, Affiliated Jinhua Hospital, Zhejiang University School of Medicine, Jinhua, 321000, China.
Mikrochim Acta. 2024 Oct 30;191(11):717. doi: 10.1007/s00604-024-06746-x.
Uniform PtCoRuRhFe high-entropy alloy nanoflowers (HEANFs) were fabricated by a simple wet-chemical co-reduction method in oleylamine for quantitative colorimetric determination of captopril (CAP) based on multi-site synergistic signal amplification. Specifically, the peroxidase mimetic activity of the PtCoRuRhFe HEANFs was examined through catalysis of 3,3',5,5'-tetramethylbenzidine (TMB) oxidation, whose catalytic mechanism was investigated by electron paramagnetic resonance (EPR) spectroscopy. The role of the ·O was figured out during the catalytic procedure. Further, the oxidation of TMB (oxTMB) can be effectively reduced by CAP, accompanied by quickly transforming the solution color from blue to colorless. More importantly, the absorbance at 652 nm is linearly related to the CAP concentration in a range 5.0-50.0 mM with a low detection limit of 2.82 mM. The method has been applied to the determination of CAP in human urine samples. It offers a simple and high-efficiency method for facile and visual detection of CAP in hospitals.
通过在油胺中使用简单的湿化学共还原方法制备了均匀的 PtCoRuRhFe 高熵合金纳米花（HEANFs），基于多部位协同信号放大，实现了对卡托普利（CAP）的定量比色测定。具体而言，通过 3,3',5,5'-四甲基联苯胺（TMB）氧化来检验 PtCoRuRhFe HEANFs 的过氧化物酶模拟活性，并通过电子顺磁共振（EPR）光谱研究其催化机制。在催化过程中确定了·O 的作用。此外，TMB 的氧化（oxTMB）可被 CAP 有效还原，伴随溶液颜色从蓝色迅速变为无色。更重要的是，在 5.0-50.0 mM 范围内，652 nm 处的吸光度与 CAP 浓度呈线性关系，检测限低至 2.82 mM。该方法已应用于人尿样中 CAP 的测定。它为医院中 CAP 的简便、可视化检测提供了一种简单高效的方法。
