Department of Dermatology, The Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi, Jiangsu, China.
Department of Neurobiology, Nanjing Medical University, Nanjing, Jiangsu, China.
Cytotherapy. 2023 Nov;25(11):1176-1185. doi: 10.1016/j.jcyt.2023.07.001. Epub 2023 Jul 29.
Extracellular vesicles and exosome-mimetic nanovesicles (NVs) derived from mesenchymal stromal cells (MSCs) have emerged as promising to promote hair growth. However, short local skin retention after subcutaneous administration hinders their clinical applications.
In this study, we prepared magnetic nanovesicles (MNVs) from iron oxide nanoparticle-incorporated MSCs. MNVs contained more therapeutic growth factors than NVs derived from naive MSCs, and their localization and internalization were manipulated by external magnetic field.
Following the subcutaneous injection of MNVs into a mouse model of depilation-induced hair regeneration, the magnetic attraction increased their skin retention. Then, the cellular proliferation and β-catenin signaling in hair follicles (HF) were markedly enhanced by MNV injection and magnetic field application. Furthermore, an acceleration of HF growth was revealed by histological analysis.
The proposed strategy can enhance the therapeutic potential of MSC-derived NVs for hair regeneration and other dermatological diseases.
间充质基质细胞（MSCs）衍生的细胞外囊泡和类外泌体纳米囊泡（NVs）在促进毛发生长方面具有很大的潜力。然而，经皮下给药后，其在皮肤中的局部滞留时间较短，限制了其临床应用。
在本研究中，我们从氧化铁纳米颗粒掺入的 MSCs 中制备了磁性纳米囊泡（MNVs）。MNVs 比来源于未成熟 MSCs 的 NVs 包含更多的治疗性生长因子，并且可以通过外部磁场来操纵其定位和内化。
将 MNVs 皮下注射到脱毛诱导的毛发生长小鼠模型中后，磁吸引作用增加了其皮肤滞留。然后，MNV 注射和磁场应用显著增强了毛囊（HF）中的细胞增殖和β-连环蛋白信号。此外，组织学分析显示 HF 生长加速。
该策略可以增强 MSC 衍生的 NVs 在毛发生长和其他皮肤疾病中的治疗潜力。
