Research and Application Center, Kastamonu University, Kastamonu, Turkey; Plantomics Research Laboratory, Department of Genetics and Bioengineering, Faculty of Engineering and Architecture, Kastamonu University, Kastamonu, Turkey.
Plantomics Research Laboratory, Department of Genetics and Bioengineering, Faculty of Engineering and Architecture, Kastamonu University, Kastamonu, Turkey; Sabancı University Nanotechnology Research and Application Center (SUNUM), Sabancı University, Turkey.
J Biotechnol. 2023 Sep 10;374:49-69. doi: 10.1016/j.jbiotec.2023.07.010. Epub 2023 Jul 28.
Heat-shock proteins (Hsps) are a family of proteins essential in preserving the vitality and functionality of proteins under stress conditions. Cucumber (Cucumis sativus) is a widely grown plant with high nutritional value and is used as a model organism in many studies. This study employed a genomics, transcriptomics, and metabolomics approach to investigate cucumbers' Hsps against abiotic stress conditions. Bioinformatics methods were used to identify six Hsp families in the cucumber genome and to characterize family members. Transcriptomics data from the Sequence Read Archive (SRA) database was also conducted to select CsHsp genes for further study. Real-time PCR was used to evaluate gene expression levels under different stress conditions, revealing that CssHsp-08 was a vital gene for resistance to stress conditions; including drought, salinity, cold, heat stresses, and ABA application. Gas Chromatography-Mass Spectrometry (GC-MS) analysis of plant extracts revealed that amino acids accumulate in leaves under high temperatures and roots under drought, while sucrose accumulates in both tissues under applied most stress factors. The study provides valuable insights into the structure, organization, evolution, and expression profiles of the Hsp family and contributes to a better understanding of plant stress mechanisms. These findings have important implications for developing crops that can withstand environmental stress conditions better.
热休克蛋白（Hsps）是一类在应激条件下维持蛋白质活力和功能的必需蛋白家族。黄瓜（Cucumis sativus）是一种广泛种植的植物，具有很高的营养价值，并且被许多研究作为模式生物。本研究采用基因组学、转录组学和代谢组学方法研究黄瓜的 Hsps 对非生物胁迫条件的反应。生物信息学方法用于鉴定黄瓜基因组中的六个 Hsp 家族，并对家族成员进行特征描述。还从序列读取存档（SRA）数据库中进行转录组数据分析，以选择 CsHsp 基因进行进一步研究。实时 PCR 用于评估不同胁迫条件下的基因表达水平，结果表明 CssHsp-08 是抵抗胁迫条件的重要基因，包括干旱、盐胁迫、冷胁迫、热胁迫和 ABA 处理。对植物提取物进行气相色谱-质谱分析（GC-MS）显示，在高温下叶片中氨基酸积累，在干旱下根部中氨基酸积累，而在施加大多数胁迫因子下，蔗糖在两种组织中积累。本研究为 Hsp 家族的结构、组织、进化和表达谱提供了有价值的见解，并有助于更好地理解植物胁迫机制。这些发现对于开发能够更好地耐受环境胁迫条件的作物具有重要意义。
